运动对去势雌性大鼠不同部位骨密度影响实验研究  

The influence of exercise on bone mineral density in ovariectomized rats

作　　者：屈强[1] 朱超[1] 彭翠宁[1] 

机构地区：[1]陕西中医学院附属医院骨科,咸阳712000

出　　处：《陕西医学杂志》2014年第11期1462-1464,共3页Shaanxi Medical Journal

摘　　要：目的:探讨运动对去势雌性大鼠不同部位骨密度(BMD)的影响。方法:将24只9月龄雌性大鼠根据体重大小进行配伍分为三组:对照组、单纯去势组、去势后运动组。单纯去势组和去势后运动组大鼠均行双侧卵巢切除。去势后运动组大鼠卵巢切除后在跑台上进行中等强度运动(18m/min,1h/d,5d/w)12周。12周后处死动物后用双能X线骨密度仪对各组大鼠椎体和股骨骨密度进行测定,以体重变化为协变量,采用协方差分析对不同部位骨密度进行分析。结果:股骨BMD组间比较差异有显著性意义,对照组明显高于去势组,运动组高于去势组和对照组,差异非常显著。腰椎BMD各组间比较差异有显著性意义,对照组和运动组均高于去势组,运动组与对照组之间无显著性差异。结论:运动可以预防去势大鼠骨量丢失,在椎体表现为预防骨量的丢失,在股骨表现为骨量增加。在大鼠骨质疏松模型中应该根据研究重点的不同选择不同的观察对象。Objective ：To investigate the influence of exercise on bone minral density of femur and lumbar in ovariectomized rats .Methods：Twenty four female rats with 9 months old were divided on the basis of body weight into 3 groups：① Control（CON）;② Ovariectomized model （OVX）;③ Exercise （Ex） .The ovarietomy was performed in OVX and Ex group .Rats in Ex group were trained on a treadmill at 18m/min for 1h/day and 5days/week for 12 weeks .Body weight was measured and animals were sacrificed at 12 weeks postoperatively .Dual‐energy X‐ray absorptiometry was carried out to determine the bone mineral density （BMD） of the femur and lumbar in both groups at 12 weeks .BMD were analysed using analysis of variance with the body weight as the covariance .Results ：There was difference in femur BMD among different groups ,BMD in CON group was significantly higher than that in OVX group ,and BMD in Ex group was higher than that in OVX and CON group（ respectively） .The difference of the BMD in lumbar between different group was significant ,BMD in OVX group was significantly lower than that in CON and Ex groups （ respectively） .No significant difference was found in BMD of lumbar between CON and Ex groups .Conclusion ：Osteopenia is detected in rats with ovaiectomy .Exercise can inhibit or reverse the osteoporosis associated with ovaiectomy ,which is more notable in femur than in lumbar .Various positon should be considered according different purpose in animal model of osteoporosis due to estrogen deficiency .

关 键 词：骨质疏松  绝经后/病理生理学 骨密度/分析 运动活动 大鼠 

分 类 号：R363[医药卫生—病理学]