葛内生真菌抑菌活性菌株的筛选及鉴定  

Identification and Antimicrobial Activity of Endophytic Fungi from Pueraria lobata

作　　者：万陕宁 麻玉萍 王家稳[1] 魏希颖[1] 

机构地区：[1]陕西师范大学生命科学学院教育部药用植物资源与天然药物化学重点实验室,西安710119

出　　处：《天然产物研究与开发》2014年第11期1879-1883,1814,共6页Natural Product Research and Development

基　　金：陕西省自然科学基金项目(2013JM3006)

摘　　要：对药用植物葛的112株内生真菌进行抑菌活性的观察,结果显示:J5-2、J100-2、J31-3这3株菌对细菌及植物病原真菌具有较好的抑制作用,其中J100-2对苹果树腐烂病菌(Valsa mali)等5种植物病原真菌均有不同程度的抑制作用,抗菌谱较广。通过形态学特征观察和rRNA基因ITS序列系统发育分析对J100-2进行鉴定,可知J100-2为半知菌门,球壳孢目,球壳孢科,茎点霉属,巨腔茎点霉(Phoma macrostoma)。A total of 112 strains of endophytie fungi were isolated from the medicinal plant Pueraria lobata,their antimierobial activity were investigated. The results showed that endophytie fungi J5-2 ,J100-2 and J31-3 had remarkable antimicrobial activities to bacteria and plant pathogenic fungi. J100-2 had varying degrees of antimicrobial activities against 5 plant pathogenic fungi including Valsa mali. It showed that J100-2 had a wide antimicrobial spectrum. J100-2 was identi- fied by its morphological features and phylogenetic analysis based on internal transcribed spacer of rRNA gene sequence comparison. Fungal morphological and molecular identification demonstrated that J100-2 represented Phoma macrostoma.

关 键 词：葛 内生真菌 抑菌活性 鉴定 

分 类 号：Q939.9[生物学—微生物学]