miRNA let-7f-1-3p在伯基特淋巴瘤中的差异性分析  

Differential analysis of miRNA let-7f-1-3p in Burkitt lymphoma

作　　者：陈宝珍[1] 师怡[1] 王晓江[1] 卢建平[1] 何银珠[1] 陈刚 

机构地区：[1]福建医科大学教学医院福建省肿瘤医院分子免疫病理研究室福建省肿瘤医学转化重点实验室,福州350014

出　　处：《白血病．淋巴瘤》2014年第10期602-606,共5页Journal of Leukemia & Lymphoma

基　　金：福建省自然基金（2012J01326）;福建省医学创新基金（2012CX7）

摘　　要：目的 研究伯基特淋巴瘤（BL）中差异表达的miRNA,探讨可用于BL鉴别诊断的miRNA标志物.方法 收集BL病例,miRNA芯片筛选其与淋巴结反应性增生之间的差异miRNA表达谱;运用miRWalk数据库对差异miRNA进行靶基因预测;利用MAS3对靶基因进行GO注释和KEGG信号通路分析.结果 与淋巴结反应性增生组织相比,BL中共有46个表达异常的miRNA,包括3 1个上调miRNA和15个下调miRNA;对miRNA let-7f-1-3p靶基因预测得到2837条靶基因,与BL密切相关的基因有16个：CENPC1、FANCF、IL4R等;GO注释显示主要涉及多细胞器官发育,RNA聚合酶Ⅱ启动子转录调控等生物学过程,KEGG分析显示主要涉及癌症信号通路,TGF-beta信号通路中.结论 在BL中发现了表达异常的miRNA,生物信息学分析对差异miRNA分析结果提示,这些差异miRNA可以作为BL鉴别诊断的新肿瘤标志物.Objective To explore the differential expression of microRNAs （miRNAs） in Burkitt lymphoma （BL） and their potential use as biomarkers for BL diagnosis.Methods The different miRNAs in BL from reactive hyperplasia of lymph node cases were screened by miRNA chip.The potential targets of miRNAs were predicted using miRWALK.MAS3 program was used to determine the putative functions of potential miRNA target genes by annotation using Gene Ontology （GO） and the Kyoto Encyclopedia of Genes and Genomes （KEGG） analyses.Results A total of 46 miRNAs （36 upregulated and 21 downregulated） were dysregulated in BL compared with reactive hyperplasia of lymph node.Interestingly,members of the let-7 family （let-7f-1-3p） was downregulated in BL.The target genes of let-7f-1-3p were predicted,and the GO enrichment analysis revealed their functions were mainly related with multicellular organismal development and regulation of transcription from RNA polymerase Ⅱ promote.KEGG pathway analysis was also carried out among the predicted target genes,which showed that they were mainly involved in TGF-beta signaling pathway and related with chronic myeloid leukemia.Conclusions The set of differentially expressed miRNAs identified here expands the range of potential diagnostic markers for BL.

关 键 词：伯基特淋巴瘤 MIRNA GO分析 KEGG分析 

分 类 号：R733.1[医药卫生—肿瘤]