TS、TP和dUTPase在大肠癌原发灶和相应淋巴结转移灶中表达差异与5-Fu化疗敏感性关系的研究  

A study for differences of expression of TS, TP and dUTPase in colorectal primary tumor and corresponding lymph node metastasis and the correlation with chemotherapy sensitive 5-Fu

作　　者：任建林 田林 赵素琴 梁建芳[2] 郑绘霞[2] 肖虹[2] 

机构地区：[1]山西省汾阳医院,032200 [2]山西医科大学第一医院

出　　处：《中国实用医刊》2014年第23期4-7,共4页Chinese Journal of Practical Medicine

基　　金：山西省卫生厅科研课题（201202027）

摘　　要：目的 观察、探讨大肠癌、切缘正常组织及其相应转移淋巴结中氟尿嘧啶类药物代谢酶胸苷酸合成酶（TS）、胸苷磷酸化酶（TP）和dUTP焦磷酸酶（dUTPase）的表达情况;研究是否存在大肠癌原发灶与相应淋巴结转移灶中TS、TP和dUTPase表达水平的差异,以及与5-氟尿嘧啶（5-ru）化疗敏感性的关系;评估TS、TP和dUTPase作为5-Fu化疗敏感性判定指标的可行性.方法 应用免疫组化方法检测33例大肠癌、切缘正常组织及相应转移淋巴结中TS、TP和dUTPase的表达情况.结果 33例大肠癌淋巴结转移灶中TS和dUTPase的表达在表达水平、染色范围和染色深度三个方面均高于原发灶,差异有统计学意义（P＜0.05）;大肠癌原发灶和淋巴结转移灶中TP的表达均为高表达,无相关性;大肠癌原发灶与淋巴结转移灶的耐药性不尽一致.结论 大肠癌原发灶和淋巴结转移灶之间的TS和dUTPase表达水平比较差异有统计学意义,应根据转移灶本身的TS和dUTPase的表达水平选择化疗方案.Objective To observe the expression of thymidylate synthase （TS）,thymidine phosphorylase（TP） and dUTPase in colorectal carcinoma,normal tissues and corresponding lymph node metastasis,to study whether there is difference in expression levels of TS,TP and dUTPase in primary tumors of colorectal carcinoma and corresponding lymph node metastasis,and the relationship with the chemosensitivity of 5-Fu,and to assess feasibility of determine indicators of TS,TP and dUTPase as 5-Fu chemosensitivity.Methods The expression of TS,TP and dUTPase in 33 cases of colorectal cancer,normal tissues and corresponding lymph node metastases were assessed by immunohistochemical method.Results The expression levels,dyeing and dyeing depth range of TS,TP and dUTPase in 33 cases of lymph node metastasis of colorectal carcinomas was higher than that in primary tumors in colorectal carcinomas,the difference was significant （P ＜ 0.05）.The expression of TP in primary tumor and lymph node metastasis in colorectal carcinomas were highly expressed,and had no correlation.The resistant in primary tumor and lymph node metastasis in colorectal cancer were not consistent.Conclusions The expression of TS and dUTPase in primary tumor and lymph node metastasis in colorectal carcinomas have significant differences,so the chemotherapy should be selected according to their metastatic expression levels of TS and dUTPase.

关 键 词：大肠癌 转移淋巴结 化疗药敏性 5-氟尿嘧啶 胸苷酸合成酶 胸苷磷酸化酶 dUTP焦磷酸酶 

分 类 号：R735.34[医药卫生—肿瘤]