马铃薯块茎形成相关基因STI-LIKE在拟南芥植株发育中的功能验证  

Functions of Potato Tuber Formation-related Gene STI-LIKE in Phenotype of Arabidopsis thaliana

作　　者：平海涛[1,2] 王玉萍[1,3] 王东霞 周晓洁[1,2] 

机构地区：[1]甘肃省作物遗传改良与创新重点实验室甘肃省干旱生境作物学国家重点实验室培育基地,兰州730070 [2]甘肃农业大学生命科学技术学院,兰州730070 [3]甘肃农业大学园艺学院,兰州730070

出　　处：《生物技术通报》2014年第11期119-124,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31060063;31260094);甘肃省自然科学基金项目(0803RJZA0510);甘肃省教育厅高校科研专项(1002-07);甘肃省财政厅高校基本科研业务费

摘　　要：马铃薯块茎的形成涉及一系列基因的表达和关闭。以马铃薯普通栽培品种"大西洋"为试验材料,采用RT-PCR扩增获得马铃薯STI-LIKE基因全长片段。RT-PCR定量分析表明,该基因在马铃薯营养器官中均有表达。生物信息学分析表明STI-LIKE蛋白具有3个TPR结构域,在高等植物中具有高同源性,是一个普遍存在的蛋白质。为验证STI-LIKE蛋白在拟南芥植株发育中功能,分别构建该基因强启动子表达载体(p BI121)和干扰载体(p HANNIBAL和p ART27),转化拟南芥获得了两种载体的拟南芥转基因植株,同时制备STI-LIKE蛋白抗体验证转基因植株蛋白表达。研究结果表明STI-LIKE基因可能参与拟南芥株型发育过程。A series of gene’s expression and silencing were involved in potato tuber formation. The full length of potatoSTI-LIKE gene was cloned by using the RT-PCR（reverse transcription PCR）method from the potato variety “Atlantic”. The expression ofSTI-LIKE gene in all potato organs were analyses by real time PCR analysis. The results showed thatSTI-LIKE gene expressed in all potato organs. The bioinformatics analysis indicated that STI-LIKE protein contains three TPR domains, and the STI-LIKE gene was homologous recombination in higher plants. The expression vector（pBI121）and interference vectors（pHANNIBAL and pART27）were constructed and transferred to Arabidopsis thaliana. The STI-LIKE protein expression level was examined by Western blot with specific antibody. The results indicated that STI-LIKE gene might be associated with the growth and development of seedling ofArabidopsis.

关 键 词：马铃薯 STI-LIKE基因 TPR结构域 株型发育 

分 类 号：S532[农业科学—作物学]