内脏脂肪素在2型糖尿病大鼠脂肪组织中的表达及意义  

Visfatin Gene in Fatty Tissue of Rats with Type 2 Diabetes Mellitus

作　　者：孙卫[1] 张敬华[2] 王文婷[1] 郑学芝[1] 念红[1] 刘桂莲[1] 

机构地区：[1]牡丹江医学院生理教研室,中国牡丹江157011 [2]牡丹江医学院附属二院,中国牡丹江157011

出　　处：《国际老年医学杂志》2014年第6期247-249,共3页International Journal of Geriatrics

基　　金：黑龙江省教育厅科研项目（11551519）

摘　　要：目的：检测实验性2型糖尿病大鼠脂肪组织中内脏脂肪素（visfatin）mRNA表达的情况，探讨visfatin与2型糖尿病相关性。方法：高糖高脂饮食喂养大鼠4周加尾静脉注射小剂量链脲佐菌素（STZ）30mg／kg复制2型糖尿病大鼠模型，继续高糖高脂饮食喂养8周后取材，测定空腹血糖（FBG）、总胆固醇（TC）、三酰甘油（TG）、血清胰岛素（FINS）并计算胰岛素抵抗指数（irt）；检测内脏脂肪组织中visfatinmRNA表达水平。结果：2型糖尿病大鼠FBG、FINS、TC、TG水平、IR值升高；内脏脂肪组织中visfatin mRNA表达量明显增高并与FBG、TG、IR呈显著正相关。结论：visfatin mRNA的表达在2型糖尿病胰岛素抵抗中起重要作用。OBJECTIVE： To observe the expression of Visfatin gene mRNA in fatty tissue of Rats （T2DM） with type 2 diabetes Mellitus and discuss the relation between visfatin and T2DM. METHODS： Wistar Rats of T2DM model were made and fed by high glucose and fat diet and injected by small dose of streptozocin （STZ） （30 mg/kg） for 4 weeks. And then high glucose and fat diet were fed to these Rats contimually for weeks. FBG, TC, TG and FINS were measured and calculate IR. Visfatin gene mRNA expres- sion in fatty tissue was determined. RESULTS： FBG, FINS, TC, TG levels and IR of T2DM rised. Visfatin gene mRNA expression increased obviously in fatty tissue of T2DM and had significantly positive correlation with FBG, TG and IR. CONCLUSION： Expres- sion of Visfatin gene mRNA play an important role in insulin resistance of T2DM.

关 键 词：2型糖尿病 内脏脂肪素 基因表达 

分 类 号：R151.2[医药卫生—营养与食品卫生学]