HPLC法同时测定川芎药材中阿魏酸和藁本内酯的含量  

Simultaneous determination of the content of ferulic acid and ligustilide in Chuanxiong by HPLC

作　　者：吕维[1] 吴恋[1] 叶娜[1] 罗红丽[1] 王春艳[1] 万丽[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2014年第2期13-15,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的：建立高效液相色谱法同时测定川芎药材中阿魏酸和藁本内酯的方法。方法：采用Dikma Kromasil C18（250nm×4．6mm，5μm）色谱柱；流动相为甲醇．0．1％冰醋酸水溶液（65：35）；流速1．0mL·min^-1。；检测波长：320nm；柱温30℃。结果：川芎药材中2种成分得到良好的分离，阿魏酸在0．032～0．3179μg围内，纂本内酯在0．244～2．44μg范围内线性关系良好，平均回收率分别为98．4％和98．8％。结论：本方法简便，准确，重复性好，为多组分评价川芎药材质量提供了可靠的分析方法。Objective： To develop a HPLC method for simultaneous determination of the content of ferulic acid and ligustilide in Chuanxiong. Method： The analysis was performed an a Kromasil C18 （250 nm×4.6 mm,5μm） column. The mobile phase consisted of methanol-0.1%glacial acetic acid（65：35）.The flow rate was 1.0 mL· min^-1. The detection wavelength was 320 nm and column temperature was 30℃. Result： The ferulic acid and ligustilide was separated perfectively. The standard curve was liner within the concentration range of 0.032μg～0.3179μg for ferulic acid and 0.244μg -2.4 μg for ligustilide. The recovery was 98.4% for ferulic acid and 98.8% for ligustilide, respectively. Conclusion： The method is simple, accurate and reproducible, which can be used for the quality control of Chuanxiong.

关 键 词：HPLC 阿魏酸 藁本内酯 川芎 

分 类 号：R284[医药卫生—中药学]