氧化苦参碱-黄芪多糖复方对新城疫Ⅳ系疫苗免疫雏鸡血清IL-2和IFN-γ水平的影响  

Effects of Oxymatrine-Astragalus Polysaccharide Compound on Levels of IL-2 and IFN-γin Chicks Vaccinated with NDV Ⅳ Vaccine

作　　者：李巨银[1] 胡新岗[1] 蔡丙严[1] 魏宁[1] 程汉[1] 桂文龙[1] 陈光明[1] 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《动物医学进展》2014年第11期119-122,共4页Progress In Veterinary Medicine

摘　　要：为了探讨氧化苦参碱-黄芪多糖中草药复方对抗新城疫病毒（NDV）感染效果，旨在为筛选确实有效的防治鸡新城疫的中药组方，将14日龄非免疫健康罗曼公雏鸡150羽随机均分为5组，1组-3组分别为氧化苦参碱-黄芪多糖复方高、中、低剂量组，4组为免疫对照组，5组为空白对照组。除空白对照组外均用新城疫Ⅳ系疫苗滴鼻、点眼免疫，28日龄重复免疫。在首次免疫的同时，1组-3组分别口服高、中、低剂量氧化苦参碱-黄芪多糖复方药物，疫苗对照组和空白对照组口服等量生理盐水，每天1次，连续3 d。分别于14、28和42日龄采血，用双抗夹心 ELISA 法检测血清中 IL-2和 IFN-γ的浓度。结果显示，复方的高、中、低剂量在各时间点血清 IL-2浓度显著高于免疫对照组（P ＜0．05）；血清 IFN-γ浓度均显著高于免疫对照组（P＜0．05）。说明中药成分复方能显著增强雏鸡对新城疫疫苗的免疫应答，高、中剂量的效果较好，可用于促进细胞免疫，提高机体抗病能力。In order to evaluate the efficacy of oxymatrine-Astragalus polysaccharide（OTM-APS）against Newcastle disease virus （NDV）infection,The prevention efficacy of compound on Newcastle disease was evaluated.One hundred and fifty 14 days old Roman cocks without vaccination were randomly divided into 5 groups.the chickens in groups 1,2 and 3 were first intranasally administrated by NDV-Ⅳ vaccine.At the same time,the cocks in groups 1,2 and 3 were orally administrated with OTM-APS compounds of high,middle and low doses,respectively.Group 4 was vaccine control and Group 5 was blank control. Cocks in group 4 was vaccinated by vaccine and taken physiological saline,once a day for three days.The cocks were boost vaccinated at 28 days old.The concentrations of IL-2 and IFN-γin the sera of cocks in the 5 groups were detected by double antibody sandwich ELISA at 14,28 and 42 days old.The results showed that the concentrations of IL-2 and IFN-γin sera of the cocks treated with medicine were significantly high-er than that of the cocks in the vaccine control group （P 〈0.05）.It indicated that OTM-APS compound could significantly strengthen the immune response of chickens to Newcastle disease vaccine and enhance the resistance against the disease..

关 键 词：氧化苦参碱-黄芪多糖复方 公雏鸡 白细胞介素-2 干扰素 双抗夹心ELISA 

分 类 号：S853.7[农业科学—临床兽医学] S852.4[农业科学—兽医学]