人宫颈癌组织和Hela细胞株中SP细胞水平和生物学特性的比较研究  

Comparison and analysis of side population cells in biological characteristics between human cervical cancer and Hela cells

作　　者：董超[1,2] 金从国[1,3] 吴星娆[1,4] 杨毅[1,4] 高碧燕[1,5] 

机构地区：[1]昆明医科大学第三附属医院 [2]云南省肿瘤医院肿瘤内二科,昆明650118 [3]云南省肿瘤医院肿瘤研究所,昆明650118 [4]云南省肿瘤医院放射治疗研究中心,昆明650118 [5]云南省肿瘤医院妇瘤科,昆明650118

出　　处：《重庆医学》2014年第32期4297-4300,共4页Chongqing medicine

基　　金：云南省卫生厅科学研究基金项目(2010NS084)

摘　　要：目的比较宫颈癌组织和Hela细胞株中多种恶性肿瘤的侧群(SP)细胞的含量和生物学特性。方法采用Altra流式细胞仪分选出宫颈癌组织原代培养6代细胞和Hela细胞株中SP细胞,检测两种细胞的表面标记和细胞周期增殖状况。结果宫颈癌组织和Hela细胞株中SP细胞水平分别为(1.62±0.48)%和(2.70±0.59)%,Hela细胞株中SP的水平明显高于宫颈癌组织,差异有统计学意义(P<0.05)。宫颈癌组织和Hela细胞株SP细胞的生物学标记(ABCG2、CD133、CD43、P63、Ki-67、MDR)表达率分别为:ABCG2(78.59±5.42)%vs.(80.17±3.60)%,CD133(51.55±6.29)%vs.(52.87±4.96)%,CD43(69.73±5.70)%vs.(70.68±5.44)%,P63(47.56±6.92)%vs.(48.06±5.28)%,Ki-67(14.69±1.69)%vs.(15.33±1.38)%,MDR(58.36±11.12)%vs.(58.24±5.32)%,表达差异均无统计学意义(P>0.05)。各细胞周期时相分布分别为:G0/G1期(94.70±1.76)%vs.(95.10±1.59)%,S期(3.25±0.99)%vs.(2.92±0.84)%,G2/M期(2.05±1.10)%vs.(1.98±0.95)%,凋亡率(4.06±0.64)%vs.(3.92±0.59)%,坏死率(1.00±0.38)%vs.(1.12±0.31)%,表达差异均无统计学意义(P>0.05)。宫颈癌组织和Hela细胞株两组SP细胞在生物学标记和细胞周期增殖状况的表达差异均无统计学意义(P>0.05)。结论宫颈癌组织和Hela细胞株中都存在SP细胞且含量不同,他们均具有肿瘤干细胞的生物学特性。Objective To compare the proportion and biological characteristics of side population cells between human cervical cancer and Hela cells.Methods Altra flow cytometry was used to isolate SP cells from human cervical cancer and Hela cells,the cells markers and cell cycles were measured.Results The proportions of SP cells in human cervical cancer and Hela cells were（1.62±0.48）% and（2.70±0.59）%,the percentage of SP in Hela cells were significantly higher than in human cervical cancer（P〈0.05）.The proportions of ABCG2,CD133,CD43,P63,Ki-67,MDR were（78.59±5.42）%vs.（80.17±3.60）%,（51.55±6.29%）vs.（52.87±4.96）%,（69.73±5.70）%vs.（70.68±5.44）,（47.56±6.92）% vs.（48.06±5.28）%,（14.69±1.69）%vs.（15.33±1.38）%,（58.36±11.12）%vs.（58.24±5.32）%in human cervical cancer and Hela cells,respectively（P〉0.05）.The proportions of G0/G1,S,G2/M were（94.70±1.76）%vs.（95.10±1.59）%,（3.25±0.99）%vs.（2.92±0.84）%,（2.05±1.10）%vs.（1.98±0.95）%,respectively.The proportions of apoptosis and necrosis were（4.06±0.64）%vs.（3.92±0.59）%,（1.00±0.38）%vs.（1.12±0.31）%.There were no significant differences in biological characteristics,cell cycle or cell apoptosis of the two SP cells between human cervical cancer and Hela cells（P〉0.05）.Conclusion Side population cells express in human cervical cancer and Hela cells,and percentages of the two SP cells are different.The two SP cells have the characteristics of cancer stem cells.So it can be viewed as the pointcut to research the cervical cancer stem cell and supply scientific basis to identify the cervical cancer stem cell.

关 键 词：宫颈肿瘤 肿瘤干细胞 侧群细胞 生物学特性 

分 类 号：R737.33[医药卫生—肿瘤]