1,25(OH)_2D_3对成骨细胞增殖及其与乳腺癌细胞黏附的影响  

Effects of 1,25(OH)_2D_3 on proliferation and differentiation of osteoblasts and influence on the adhesion of breast cancer cells and the osteoblasts

作　　者：吴玉[1] 卫红艳[1] 翟琼莉[2] 

机构地区：[1]天津医科大学总医院,天津300052 [2]天津医科大学肿瘤医院,国家肿瘤临床医学研究中心,中美淋巴血液诊治中心,天津市肿瘤防治重点实验室

出　　处：《山东医药》2014年第39期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81272361)

摘　　要：目的：观察1，25二羟基维生素D3[1，25（OH）2D3]对成骨细胞增殖及其与乳腺癌细胞黏附的影响。方法取对数生长期人成骨细胞hFOB1．19，随机分为三组，正常对照组用DMEM/F12培养基培养；溶剂对照组用含0．1mL/L乙醇的DMEM/F12培养基培养；1，25（OH）2D3组分别用含1×10^-7、1×10^-8、1×10^-9、1×10^-10mol/L1，25（OH）2D3的DMEM/F12培养基培养。1-3d后于酶标仪上450nm处测定吸光度值，计算细胞增殖抑制率；采用微量酶标仪法检测hFOB1．19细胞培养上清中碱性磷酸酶（ALP）活力。取对数生长期人乳腺癌细胞MDA-MB-231、T47D，分组及处理同上，各组培养24h后加入对数生长期hFOB1．19细胞，采用细胞黏附法计算MDA-MB-231或T47D与hFOB1．19细胞黏附率。结果正常对照组、溶剂对照组及1×10^-7、1×10^-8、1×10^-9、1×10^-10mol/L1，25（OH）2D3组细胞增殖抑制率第3天分别为0、-1．63％±2．04％、16．02％±1．13％、10．78％±0．98％、8．82％±2．59％、5．55％±0．57％，各组间比较，P均＜0．05；ALP活力分别为（1．77±0．03）、（1．76±0．01）、（2．11±0．02）、（1．96±0．01）、（1．84±0．04）、（1．81±0．03）金氏单位/100mL，1×10-7、1×10-8moL/L1，25（OH）2D3组与其他四组比较，P均＜0．05；MDA-MB-231与hFOB1．19细胞黏附率分别为58．80％±3．11％、59．80％±3．96％、42．00％±1．87％、46．40％±2．30％、53．80％±0．84％、57．00％±3．24％，T47D与hFOB1．19细胞黏附率分别为55．75％±2．22％、56．25％±1．71％、47．80％±3．11％、50．60％±2．07％、54．40％±2．70％、57．20％±1．48％，1×10^-7、1×10^-8moL/L1，25（OH）2D3组与其他四组比较，P均＜0．05。结论1，25（OH）2D3能抑制成骨细胞增殖，促进成骨细胞分化，降低乳腺癌细胞与成骨细胞的黏附。Objective To investegate the effect of 1,25（OH）2D3 on osteoblastic proliferation and differentiation and to detecte the influence of 1,25（OH）2D3 on the adhesion of breast cancer cells and the osteoblasts.Methods The human osteolasts（hFOB1.19） were divided into conrol group, ethanol group and 1,25（OH）2D3 groups which were cultured and treated in normal DMEM/F12 medium, DMEM/F12 medium with 0.1 ml/L ethanol and DMEM/F12 medium with different concentrations of 1,25（OH）2D3 respectively.The cellular proliferation inhibition rate of human osteoblasts was measured by CCK-8 assay.The ALP activity was measured by trace enzyme assay.The adhesion of human breast cancer cell lines and hFOB1.19 were detected by cell adhesion method.Results The proliferation inhibition rate of osteoblasts cultured in control group, ethanol group or 1,25（OH）2D3 groups whoes concentration was 1 ×10 ^-7, 1 ×10^ -8, 1 ×10^ -9, 1 ×10^ -10 mol/L were 0, -1.63%±2.04%, 16.02%±1.13%, 10.78%±0.98%, 8.82%±2.59%, 5.55%±0.57%respec-tively（P〈0.05） at the third day.The ALP activity in each group was （1.77 ±0.03）, （1.76 ±0.01）, （2.11 ±0.02）, （1.96 ±0.01）, （1.84 ±0.04）, （1.81 ±0.03） King unit/100 mL respectively.The adhesion of MDA-MB-231 and hFOB1.19 cells cultured in each group was 58.80%±3.11%, 59.80%±3.96%, 42.00%±1.87%, 46.40%±＆amp;nbsp;2.30%, 53.80%±0.84%, 57.00%±3.24% respectively.The adhesion of T47D and hFOB1.19 cells cultured was 55.75%±2.22%, 56.25%±1.71%, 47.80%±3.11%, 50.60%±2.07%, 54.40%±2.70%, 57.20%±1.48%respectively.There were statistically significant in the ALP activity, adhesion of MDA-MB-231 and hFOB1.19 cells cul-tured, adhesion of T47D and hFOB1.19 cells cultured among 1,25（OH）2D3 groups whoes concentration was 1 ×10 ^-7, 1 ×10^ -8 with other groups.Conclusions 1,25（OH）2D3 can inhibit the proliferation of osteoblasts and promote the activi-ty of ALP.It can also inhibit the cell adhesion of breast cancer cells and osteoblast cells.

关 键 词：乳腺癌 乳腺肿瘤 骨转移 1 25二羟基维生素D3 细胞增殖 细胞黏附 碱性磷酸酶 1 25(OH)2D3 

分 类 号：R737.9[医药卫生—肿瘤]