人类白细胞抗原HLA-DQA1基因多态性在尘肺肺癌患者中的表达  

Expression of HLA-DQA1 gene polymorphism in the lung cancer patients with pneumoconiosis

作　　者：赵娜[1,2] 杨锡贵[1,2] 曹殿凤[3] 高萍[3] 张宝玲[3] 

机构地区：[1]山东省医学科学院山东省肿瘤医院内科,济南250017 [2]济南大学山东省医学科学院医学与生命科学学院 [3]淄博市职业病防治院

出　　处：《国际肿瘤学杂志》2014年第10期779-781,共3页Journal of International Oncology

摘　　要：目的 检测人类白细胞抗原（HLA） DQA1位点基因多态性在尘肺肺癌患者与非职业性肺癌患者的表达差异.方法 用多重聚合酶链反应-连接酶检测反应分型法（PCR-LDR）,分别检测21例尘肺并发肺癌患者及40例非职业性肺癌患者的HLA-DQA1共6个位点的等位基因,并分析其和尘肺肺癌发病的相关性.结果 尘肺肺癌组患者HLA-DQA1 0301基因频率19％,非职业性肺癌组患者HLA-DQA1 0301基因频率2.5％,差异有统计学意义（χ2=10.022,P=0.002）,其余等位基因两组差异无统计学意义.结论 HLA-DQA1的基因多态性可能与尘肺肺癌易感性相关.DQA1 0301是尘肺并发肺癌的重要的调控基因之一,可能为尘肺肺癌易感的危险因素.Objective To detect the difference of the expression of HLA-DQA1 genes polymorphism in the lung cancer patients with pneumoconiosis and the non-occupational lung cancer patients. Methods Multi- ple polymerase chain reaction-ligase detection reaction classification method （PCR-LDR） was used to detect 6 alleles in HLA-DQA1 and the expression of HLA-DQA1 gene between the 21 cases of pneumoconiosis compli- cated with lung cancer and 40 cases of non-occupational lung cancer respectively. The correlation between them with pneumoeoniosis complicated with lung cancer was analysed. Results HLA-DQA1 0301 gene frequencies were 19% in pneumoconiosis complicated with lung cancer group and 2.5% in non-occupational lung cancer group respectively, with a statistically significant difference （χ2 = 10. 022, P = 0. 002）. There was no statisti- cally significant difference in the other allele between the two groups. Conclusion HLA-DQA1 gene polymor- phism may be related to the susceptibility of the pneumoconiosis complicated with lung cancer. DQA1 0301 is one of the important genes of pneumoconiosis complicated with lung cancer, which maybe the risk factor for the susceptibility of the pneumoconiosis complicated with lung cancer.

关 键 词：尘肺 肺肿瘤 基因多态性 

分 类 号：R734.2[医药卫生—肿瘤] R135.2[医药卫生—临床医学]