丁酸钠对肝性脑病大鼠血氨浓度的影响初探  

Study on effect of sodium butyrate on blood ammonia concentration of hepatic encephalopathy in rats

作　　者：张强[1] 焦晨阳 李根茹 葛长青[1] 张玲[1] 林维佳[1] 王珍[1] 李毓[1] 李丹丹[1] 徐传英[2] 孙红[3] 

机构地区：[1]徐州医学院临床医学系,江苏徐州221004 [2]徐州医学院病理生理教研室,江苏徐州221004 [3]徐州医学院生理教研室,江苏徐州221004

出　　处：《中国肝脏病杂志（电子版）》2014年第3期13-16,共4页Chinese Journal of Liver Diseases：Electronic Version

基　　金：江苏省大学生创新性训练计划立项项目(2012JSSPITP1820)

摘　　要：目的观察不同浓度丁酸钠对肝性脑病(HE)大鼠血氨浓度的影响以及对HE的防治效果。方法采用腹腔注射硫代乙酰胺(TAA)诱导急性肝性脑病模型。饲喂两周后造模两天,观察大鼠一般情况,并进行神经反射评级,血浆TBil、DBil、AST、ALT、血氨和肠道p H值等指标的测定。结果丁酸钠各组HE大鼠TBil、DBil、ALT、AST均有所下降,其中丁酸钠A组的肠道p H值较模型组差异有统计学意义(P<0.05),丁酸钠A、B两组的血氨浓度较模型组明显降低(P均<0.001)。丁酸钠处理可改善大鼠的神经反射,降低大鼠肝性脑病的分期。结论丁酸钠通过酸化肠道,降低HE大鼠的血氨浓度,进而改善HE大鼠所表现出来的精神症状。Objective To observe the effect of ammonia concentrations and the preventing and treating effects on hepatic encephalopathy （HE） in rats by different concentrations of sodium butyrate. Methods The hepatic encephalopathy rat models were induced by intraperitoneal injection thioacetamide （TAA） after fed two weeks made mould for two days. General condition and the nerve reflection, the level of TBil, DBil, ALT, AST, NH3 in serum, the intestinal pH values and liver histopathology were detected. Results TBil, DBil, ALT, AST of each sodium butyrate groups were decreased. The intestinal pH value of sodium butyrate group A compared with model group was statistically significant （P 〈 0.05）, blood ammonia concentrations of sodium butyrate group A and group B were significantly lower than that in model group （P 〈 0.001）. In addition, sodium butyrate treatment can improve neuro-reflex, decrease the staging of HE. Conclusions The results indicated that the ammonia concentrations can be reduced because of the souring of the intestinal tract by sodium butyrate, which could also improve the psychiatric symptoms of the hepatic encephalopathy.

关 键 词：肝性脑病 硫代乙酰胺 丁酸钠 血氨浓度 

分 类 号：R575.3[医药卫生—消化系统]