αV,β3整合素在绵羊体外受精胚胎的表达模式研究  

The Expression Pattern of αV,β3 Integrin on Ovine Embryo Derived From in Vitro Fertilization

作　　者：万鹏程[1,2] 石文艳[3] 代蓉[1,2] 周平[1,2] 石国庆[1,2] 曾申明[4] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [2]新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000 [3]石河子大学医学院生理教研室,新疆石河子832000 [4]中国农业大学动物科技学院,北京100193

出　　处：《新疆农业科学》2014年第9期1711-1717,共7页Xinjiang Agricultural Sciences

基　　金：国家科技支撑计划(2011BAD28B05);"863"计划(2011AA100307);国家星火计划项目(2012GA891001);国家自然科学基金(31160460);新疆兵团绵羊繁育生物技术重点实验室开放课题(2013KLS02);兵团院士资金专项(2011BB024);新疆兵团育种攻关(2011BA006)

摘　　要：【目的】探讨胚胎附植调控因子αV,β3整合素在绵羊早期体外胚胎的表达情况,为早期胚胎发育及附植调控机理提供理论依据。【方法】采用RT-PCR和间接免疫荧光染色技术,检测绵羊体外受精个发育阶段胚胎中αV及β3整合素mRNA的表达模式及蛋白表达定位情况。【结果】在绵羊2-细胞至桑椹胚体外胚胎中未能检测到整合素αV及β3 mRNA的表达,但可以检测到囊胚期胚胎有微量整合素αV及β3 mRNA的表达。并且在绵羊第7 d扩张囊胚和第8 d孵化囊胚上均可检测到整合素αVβ3蛋白的表达,且蛋白表达主要分布在胚胎滋养层细胞周围,孵化囊胚蛋白表达量略高于扩张囊胚。【结论】αV,β3整合素在绵羊早期胚胎发育及附植过程中具有重要调控作用。[Objective and Method] To investigate the expression pattern of integrin αV,β3 subunits in ovine preimplantation embryos,we detected the mRNA expression and localization of integrin αV,β3 subunits in ovine in vitro fertilization （IVF） embryos during different development stages using RT-PCR and indirect immune fluorescence staining technique at present research.[Result] The results showed that the expression of αV and β3 subunits mRNA could be detected in ovine IVF blastocysts in days 7-8 except at other development stages.Moreover,the protein of αVβ3 was also expressed in expended blastocysts at day 7 and hatched blastocysts at day 8,and the protein of αVβ3 was mainly distributed t around of trophoblastic cell.[Conclusion] αVβ3 is one of the role regulation factors during peri-implantation in ovine.

关 键 词：ΑVΒ3 胚胎 绵羊 

分 类 号：S826[农业科学—畜牧学] S188[农业科学—畜牧兽医]