肥胖相关基因FTO表达水平与2型糖尿病的相关性  

The correlation of the expression levels of obesity-associated gene FTO and type 2 diabetes

作　　者：王小红[1] 杨威[1] 李珊[1] 郑丽丽[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052

出　　处：《中华临床医师杂志（电子版）》2014年第20期15-19,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨肥胖相关基因FTO表达水平与2型糖尿病的相关性。方法选取河南地区135例2型糖尿病患者,分为肥胖组(60例)与非肥胖组(75例),郑州大学第一附属医院体检科体检的正常人(51例)作为正常对照组,以RT-PCR法测定FTO mRNA表达水平,ELISA法测定FTO蛋白表达水平,分析FTO表达水平与2型糖尿病患者的相关性。结果 2型糖尿病两组的FTO mRNA及蛋白表达水平高于正常组(P<0.05);FTO表达水平与收缩压、舒张压、FINS、HOMA-IR、FBG、2 h BG、HbA1c、24 h Alb、TC呈正相关(P<0.05),与HDL-C呈负相关(P<0.05),而与BMI无关(P>0.05);Logistic回归分析显示:BMI、TG、HOMA-IR、FTO基因及蛋白表达水平是患2型糖尿病的主要危险因素。结论新诊断2型糖尿病患者外周血淋巴细胞FTO基因和血清FTO蛋白高表达,可能是2型糖尿病的早期预测因子之一。Objective To explore the correlation of the expression levels of the fat mass and obesity-associated gene（FTO） and type 2 diabetes. Methods 135 cases with type 2 diabetes mellitus in Henan province were selected as experimental subjects, divided into obese group（n=60 cases） and non-obese group （n=75 cases）, and selected people who were diagnosed as normal person in the department of physical examination as control group （51 cases）. Meanwhile the expression level of FTO mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR）, and the expression level of FTO protein was measured by enzyme-linked immunosorbent assay （ELISA）, and then, analysing the correlation between the expression level of FTO gene and clinical parameters of type 2 diabetes. Results The expression level of FTO gene in obese and non-obese group of type 2 diabetic patients were higher than normal group （P〈0.05）; and FTO mRNA and protein expression level were positively correlated with systolic blood pressure, diastolic blood pressure, fasting insulin （FINS）, homeostasis model insulin resistance index （HOMA-IR）, fasting blood glucose （FBG） and 2 h BG, HbA1C, 24 h Alb and TC （P〈0.05）, but were inversely related to HDL-L （P〈0.05）, and had no relationship with BMI. Logistic regression analysis showed：BMI, TG, HOMA-IR, the expression levels of FTO gene and FTO protein were the major risk factors for type 2 diabetes. Conclusion The FTO expression levels of newly diagnosed type 2 diabetes is significantly increased, FTO gene may be one of early predictors of type 2 diabetes.

关 键 词：糖尿病 2型 肥胖症 基因 FTO 

分 类 号：R587.1[医药卫生—内分泌] R589.2[医药卫生—内科学]