Cyclin D1在结缔组织生长因子诱导肺动脉平滑肌细胞增殖中的作用  

The role of Cyclin D1 in CTGF-induced the proliferation of pulmonary artery smooth muscle cell

作　　者：李刚[1] 夏纪毅[2] 宋雪琴[3] 刘斌[1] 赵健[1] 贾鹏[1] 

机构地区：[1]泸州医学院附属医院儿二科,四川泸州64600 [2]泸州医学院药物与功能性食品研究中心,四川泸州64600 [3]泸州医学院附属医院中心实验室,四川泸州64600

出　　处：《泸州医学院学报》2014年第5期492-495,共4页Journal of Luzhou Medical College

摘　　要：目的：探讨Cyclin D1在结缔组织生长因子（CTGF）促进肺动脉平滑肌细胞（PASMC）增殖中的作用。方法：分离并培养SD大鼠肺动脉中膜平滑肌细胞,实验使用第3~7代细胞。经鉴定的PASMCs给予浓度分别为5 ng/ml、10 ng/ml、25 ng/ml、50 ng/ml、100 ng/ml CTGF培养48 h。采用WST-1检测细胞增殖;实时荧光RT-PCR和Western blot方法检测Cyclin D1m RNA及蛋白的表达。结果：随着CTGF浓度的增加,各组PASMCs的OD值均逐渐升高,当浓度达到5 ng/ml时与对照组比较,差异有统计学意义（P〈0.05）,呈浓度依赖性;随着CTGF浓度的增加,各组PASMCs的Cyclin D1 m RNA和蛋白均较对照组表达升高。结论：CTGF通过上调Cyclin D1表达促进PASMC增殖。Objective：To investigate the role in Cyclin D1 in pulmonary artery smooth muscle cells（PASMCs）induced by connective tissue growth factor（CTGF）. Methods： The PASMCs isolated from the pulmonary artery of SD rat were cultured in M199 culture system in vitro and the 3rd ~7th generation of PASMCs were used in the experiments. The cultured PASMCs were treated with 5 ng/ml, 10 ng/ml, 25 ng/ml, 50 ng/ml, 100 ng/ml CTGF,respectively. The proliferation of PASMCs was detected by WST-1 assay. The expression of Cyclin D1 was detected by real-time fluorescence RT-PCR and Western blotting. Results： The results of WST-1 test showed that, OD value in each group was gradually elevated with the increasing concentration of CTGF; when 5 ng/ml CTGF was used, the difference had statistical significance compared with the control group, which was concentration dependent. With the increase of CTGF concentration, both m RNA and protein expression of Cyclin D1 in each group were higher than that in the control group. Conclusions： CTGF may induce PASMC proliferation via up-regulated the expression of cyclin D1.

关 键 词：结缔组织生长因子 细胞增殖 CYCLIN D1 肺性高血压 

分 类 号：Q253[生物学—细胞生物学]