海南粗榧内生真菌BWL6007的鉴定及抗菌活性研究  

Identification and antimicrobial activity of endophytic fungus BWL6007 from Cephalotaxus hainanensis Li.

作　　者：吴延春[1] 刘四新[1] 蒋春洁[1] 齐静[1] 李从发[1] 胡晓苹[1] 李武[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《广东农业科学》2014年第18期110-113,共4页Guangdong Agricultural Sciences

基　　金：科技部中泰长期合作研究开发项目(18-514J);中国博士后科学基金(90279);海南省自然科学基金(313034)

摘　　要：应用传统真菌形态学鉴定方法,对海南粗榧内生真菌BWL6007进行鉴定。以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、水稻纹枯病菌、油菜菌核病菌、香蕉枯萎病菌、镰刀菌、罗非鱼源无乳链球菌为测试菌种,对海南粗榧内生真菌BWL6007在不同生长周期15 d内的发酵液抗菌活性进行测定。然后,依次采用石油醚、二氯甲烷、乙酸乙酯、三氯甲烷和正丁醇对发酵液进行分部提取,再测定各部粗提物的抗菌活性。结果表明,海南粗榧内生真菌BWL6007属于生赤壳属(Bionectria),是首次从海南粗榧中分离获得的生赤壳属内生真菌;其最佳发酵时间为11 d;发酵液乙酸乙酯部提取物的抗菌效果最佳,活性成分主要以中极性的物质为主。An endophytic fungus BWL6007 from Cephalotaxus hainanensis Li. was identified by the traditional morphological identification method. The antimicrobial activities of the fermentation broth at different fermentation time of the strain BWL6007 were determined using Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Rhizoctonia solani,Sclerotinia sclerotiorum, Fusarium oxysporum f.sp. cubense, Fusarium oxysporum f.sp.cubense FJAT-370 as the test microorganisms, and also the antimicrobial activities of extracts of various solvents were determined. The results showed that the fungus was identified as Bionectria sp., which was first time isolated from C. hainanensis Li.; the best fermentation time was 11 d; and the antimicrobial active ingredients were in ethylacetate extracts.

关 键 词：海南粗榧 抗菌活性 生赤壳属 

分 类 号：S567.1[农业科学—中草药栽培]