市场海产品铅含量分析以及小鼠对铅的吸收研究  

The Study of Lead Content in Market Seafood and Their Absorption in Mice

作　　者：童永彭[1] 郝昕[1] 罗奇[1] 张宇[1] 普学乐 孙慧斌[1] 何伟彪 何龙 陈建敏[3] 

机构地区：[1]深圳大学物理科学与技术学院,广东深圳518060 [2]深圳市环境监测中心站,广东深圳518049 [3]安捷伦科技(中国)有限公司,上海200080

出　　处：《广东微量元素科学》2014年第10期21-26,共6页Trace Elements Science

基　　金：深圳科创委资助"深圳儿童铅摄入主要途径的追踪研究"(No.JC201005280550A);国家自然科学基金面上项目(11375117);上海光源国家实验室开放课题(08sr0107)资助

摘　　要：用电感耦合等离子体质谱（ICP -MS）测量了深圳市市场上销售的花甲、生蚝、鲍鱼等16种海产品，分析了铅含量以及同位素比，其中响螺、青口贝、生蚝的铅质量分数最高，分别为（1．52±0．21），（1．66±0．23），（1．16±0．15）μg/g 干质量，超标，其它小于1．00μg/g 干质量，同位素比207 Pb/206 Pb 为0．76～0．89之间。将花甲，鲍鱼，生蚝，喂食昆明小白鼠（每组5只，质量35～38 g），喂食前后（24 h）眼球取血0．030 mL 左右对比分析海鲜中铅进入血液的量，结果表明，生蚝喂食后铅有明显的上升，其中孕鼠生物有效性显著大于对照鼠。通过用水合醋酸铅加入鼠粮中喂食哺育期间母鼠，而被哺乳的新生小鼠血铅含量和母鼠喂食铅成正比，最后一组小鼠血液铅达到（1．95±0．35）μg/g，该组一直喂养2个月（8周）后小鼠个体体质量（15±4 g）与对照（26±5 g）相比显著小。实验表明高含量铅的海产品对孕妇以及哺乳期间母亲和婴儿更为危险。By ICP -MS （Inductively coupled plasma mass spectrometry）, the lead content as well as their isotope ratios of 16 kinds of seafood in shenzhen market were measured.The lead content in paphiaundulata, green mussel, oysters, are the highest of those seafood.They were 1.52 ±0.21, 1.66 ±0.23 and 1.16 ±0.15 μg/g dry weight respectively （which is exceeded the standard 1.00 μg/g）. Their isotope ratio 207 Pb/206 Pb was between 0.76 ~0.89.The paphiaundulata, oysters, abalone were selected to feed kunming mice （n =6, heavy 35 ~38 g）.Before and after feeding （24 hours） around, 0.030 mL blood from the mice eye was analyzed bu ICP -MS.The results show that after feeding oysters lead bioavailability significantly increased in pregnant mice.By adding hydrated lead acetate in rats fed grain, feeding pregnant mice during the suckling, blood lead in newborn mice proportional to the feedinglead, and the last group of mice to reach the lead in blood 1.95 ±0.35 μg/g.After two months （8 weeks） fed the weight of this group （15 ±5 g）, was significantly lower than that of control （26 ±5 g）.This experiments show that the lead is passed to the mice through breast -feeding, pregnant rats with high lead early can lead to poor mouse lifelong developmental, individual small.Thus high levels of lead in seafood for pregnant women, infants and lactating mothers is more dangerous.

关 键 词：电感耦合等离子体质谱分析 铅 海产品 

分 类 号：O657.31[理学—分析化学]