枯草芽孢杆菌转化人参总苷为Rg_3的研究  

Microbial transformed ginsenoside Rg_3 from total saponins of Panax ginseng by Bacillus subtilis

作　　者：陈旸[1] 张美萍[1] 王义[1] 孙亮[1] 王康宇[1] 蒋世翠[1] 孙春玉[1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118

出　　处：《时珍国医国药》2014年第11期2676-2678,共3页Lishizhen Medicine and Materia Medica Research

基　　金：吉林省科技发展计划项目(No.20120917)

摘　　要：目的探索枯草芽孢杆菌发酵人参的最佳工艺,将人参总苷转化成稀有人参皂苷Rg3。方法通过正交设计软件对发酵培养基、发酵温度、发酵p H值、发酵时间进行4因素4水平的正交试验设计,比色法测定发酵前后人参总苷含量,HPLC法测定发酵前后人参皂苷Rg3的含量。结果枯草芽孢杆菌对人参发酵2天时,人参总苷含量增加19.8%,人参皂苷Rg3含量是未发酵人参的2.3倍。结论建立的发酵体系工艺合理,对稀有人参皂苷Rg3的制备具有重要意义。Objective To explore Ginseng fermentation process by Bacillus subtilis and make total saponins transformed into rare ginsenoside Rg3. Methods Make a design of L16（4^4） orthogonal test which include fermentation medium,temperature,p H value and training time,a total of 4 factors,determination of total saponins content by colorimetric method,determination of ginsenoside Rg3 by HPLC method. Results Fermentation on 2 days with Bacillus subtilis,the content of total saponins was increased by19. 8%,the content of ginsenoside Rg3 was increased 1. 3-fold. Conclusion The fermentation system＇s process was reasonable,important significance for rare ginsenoside Rg3 preparation.

关 键 词：人参 发酵 枯草芽孢杆菌 人参皂苷RG3 

分 类 号：R284.1[医药卫生—中药学]