诱导型狨猴肝纤维化模型的建立  

Establishment of drug-induced common marmoset model for hepatic fibrosis

作　　者：李萌乾 王青松[1,2] 郭正隆 尹海芳[1,2] 

机构地区：[1]天津医科大学基础医学研究中心 [2]天津医科大学细胞生物学系,天津300070

出　　处：《天津医科大学学报》2014年第6期433-436,共4页Journal of Tianjin Medical University

基　　金：国家973重大科学研究计划资助项目(2012CBA01305)

摘　　要：目的:建立诱导型狨猴肝纤维化模型。方法:手术切除普通狨猴部分肝脏,通过腹腔注射二乙基亚硝胺(DENA-2.5 mg/kg),并利用体质量和肝功能检测、病理分析、免疫组化,胶原染色和B超小动物活体成像等手段进行监测。结果:手术切除肝脏促使狨猴肝脏再生,并伴随肥大和炎症反应;DENA药物诱导会引发与人类类似的肝脏炎症,肝脏胶原聚集和血管增生,最终导致狨猴肝脏纤维化。结论:手术切肝联合DENA化学诱导可以成功获得狨猴肝纤维化模型。Objective： To establish the drug-induced common marmoset model for hepatic fibrosis. Methods： Hepatectomy and intraperitoneal injection of DENA at the dose of 2.5 mg/kg was used and various assays were performed to monitor the bodyweight, liver function, pathology, and collagen in common marmosets. Furthermore, non-invasive imaging systems were applied i.e. B ultrasound and live animal imaging. Results： The generation of newborn liver and inflammation was accelerated by hepatectomy. DENA was effective in inducing hepatitis, vascular proliferation, accumulation of collagen and fibrosis as observed in clinic. Conclusion： The common marmoset model for hepatic fibrosis has been successfully established with the combination of hepatectomy and DENA.

关 键 词：肝纤维化 普通狨猴 二乙基亚硝胺 肝切除 

分 类 号：R575.2[医药卫生—消化系统]