远志皂苷元对新生大鼠海马神经干细胞分化的影响  

Effect of Tenuigenin on Differentiation of Neural Stem Cells from Hippocampus of Newborn Rats

作　　者：陈玉静[1] 黄小波[1] 陈文强[1] 王宁群[1] 

机构地区：[1]首都医科大学宣武医院中医科,北京市100053

出　　处：《中国康复理论与实践》2014年第11期1028-1030,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81102624)

摘　　要：目的观察远志皂苷元对体外培养的胎鼠海马神经干细胞(NSCs)增殖分化的影响。方法利用无血清DMEM/F12体外培养技术从新生Wistar大鼠大脑海马中培养NSCs,添加不同浓度(0、1、2、4μg/ml)远志皂苷元。应用免疫荧光技术对NSCs及其分化后的细胞进行鉴定。结果培养的NSCs能够表达Nestin,并具有分化为神经元、星形胶质细胞及少突胶质细胞的能力。在同一接种密度条件下,远志皂苷元干预组的Nestin阳性细胞数较对照组减少(P<0.05),神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP)阳性细胞数较对照组增加(P<0.05)。结论远志皂苷元能促进新生大鼠大脑海马NSCs的分化。Objective To observe the effect of tenuigenin on differentiation of hippocampal neural stem cells （NSCs）. Methods NSCs isolated from newborn （within 24 h） Wistar rats hippocampus were cultivated in vitro with serum free and clone culturing technology. Tenui-genin of different doses （0, 1, 2, 4μg/ml） were added in the medium, and the proliferation and differentiation of the cells were identified with immunofluorescence staining. Results The neural spheres obtained from the hippocampi of newborn rats were positive for Nestin ex-pression, with the potential for further cloning and differentiation into neurons or glial cells. The incidence of neuron specific enolase and glial fibrillary acidic protein positive cells increased in all the tenuigenin groups compared to the control （P〈0.05）, while the Nestin positive cells decreased （P〈0.05）. Conclusion Tenuigenin may promote the differentiation of neural stem cell into nerve cell.

关 键 词：远志皂苷元 神经干细胞 细胞培养 细胞分化 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]