JAK2,HSP基因蛋白表达与卵巢癌紫杉醇化疗耐药相关性的研究  

Study on the relationship of JAK2,HSP protein expression and paclitaxel resistance of ovarian cancer

作　　者：李健[1] 李红霞[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院,北京100038

出　　处：《中国优生与遗传杂志》2014年第11期25-26,43,F0002,共4页Chinese Journal of Birth Health & Heredity

基　　金：国家"十一五"863计划基金资助项目(2006AA02A245)

摘　　要：目的检测卵巢癌细胞株及紫杉醇化疗后卵巢癌组织中JAK2,HSP,基因蛋白表达的变化,探讨其与卵巢癌紫杉醇化疗耐药的关系及临床意义。方法选用卵巢癌紫杉醇耐药细胞株SKOV3/TAX及敏感细胞株SKOV3,及58例卵巢癌组织,包括卵巢癌术后及紫杉醇化疗后复发患者19例(化疗组),术前未化疗患者39例(未化疗组)。采用免疫组化二步法,检测卵巢癌组织及细胞中JAK2,HSP基因的蛋白表达。结果 1.光镜下观察SKOV3/TAX细胞株中JAK2、HSP蛋白表达强度均明显高于SKOV3细胞株;组织中化疗组与未化疗组相比JAK2,HSP的蛋白表达累积光密度值均明显增加(分别为:36987.38±16873.42 vs 27756.29±7136.09;34905.64±12361.87 vs 26684.56±12662.24),差异均有统计学意义(P<0.05)。2.各基因蛋白表达在临床分期,分化程度和病理类型中均无显著性差异。3.JAK2、HSP基因蛋白表达两者间呈正相关关系(r=0.330,P=0.011)。结论 JAK2、HSP基因蛋白表达上调可能与卵巢癌紫杉醇化疗耐药有一定相关性。Objective：To investigate the expression of JAK2,HSP protein in Ovarian cancer tissues and cells after taxolchemotherapy,Explore the relationship of taxol resistant in ovarian cancer and their clinical significance. Methods：58 cases of ovarian cancer tissue were selected,among them,19 cases have received taxol-based chemotherapy previously（group 1）,and 39 cases never received chemotherapy before operation（group 2）. Ovarian cancer cell line SKOV3 and paclitaxel resistant cell line SKOV3 / TAX were selected. The expression of JAK2,HSP protein in tissues and cells were deteced by using Twostep immunohistochemical method. Results：1. The protein expression intensity of JAK2,HSP in cell line SKOV3 / TAX was significantly higher than that in the cell line SKOV3. The integrated option density（IOD）of JAK2,HSPprotein in group1 were significantly higher than those in group 2（36987.38±16873.42 vs 27756.29±7136.09;34905.64±12361.87 vs26684.56±12662.24 respectively,both P〈0.05）2. No significant difference of 2 protein expression were detected among histological types,pathological grade,and clinical-pathological stage. 3. There was a positive correlation between the expression of JAK2 and HSP protein（r=0.330,P=0.011）. Conclusions：The up-regulation of JAK2 and HSP genes are related to the mechanism of paclitaxel resistance in ovarian cancer.

关 键 词：JAK2 HSP 卵巢癌 紫杉醇耐药 

分 类 号：R737.31[医药卫生—肿瘤]