茶叶中黄曲霉毒素的高效液相色谱串联质谱法  

Determination of aflatoxins in tea by high performance liquid chromatography tandem mass spectrometry

作　　者：王可[1] 魏永巨[2] 何燕[1] 曹丽玲[1] 左书梅[1] 

机构地区：[1]石家庄市疾病预防控制中心理化检验所,河北050011 [2]河北师范大学化学与材料科学学院

出　　处：《环境与健康杂志》2014年第8期718-720,共3页Journal of Environment and Health

基　　金：石家庄市科学技术研究与发展计划(121461803)

摘　　要：目的建立茶叶中黄曲霉毒素B1、B2、G1和G2的高效液相色谱串联质谱（HPLC-MS/MS）法。方法样品经70%甲醇超声提取15 min,免疫亲和柱净化后,用ZORBAX Eclipse Plus C18色谱柱（2.1 mm×100 mm,1.8μm）进行分离,流动相为甲醇-0.1%甲酸水（体积比为45∶55）,采用多反应监测（MRM）正离子模式进行检测。结果在0.5~50.0 ng/ml的线性范围内,所得4种黄曲霉毒素的回归方程均具有良好的线性关系（r≥0.999 5）。该方法检出限为0.02~0.04μg/kg,定量下限为0.07~0.13μg/kg;平均回收率为75.6%~98.5%,RSD为3.9%~7.1%。结论该方法简单、准确、灵敏度高,适用于茶叶中黄曲霉毒素B1、B2、G1和G2的批量检测。Objective To establish a method for the determination of aflatoxins B1,B2,G1 and G2in tea by high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS）. Methods The samples were extracted ultrasonically for 15 min by 70% methanol,then purified by immunoaffinity column. The analytes were seperated by ZORBAX Eclipse Plus C18column（2.1 mm×100 mm,1.8 μm）,eluted with methanol-0.1% formic acid solution（45∶55,V/V）,then determined by HPLCMS/MS with multiple reation monitoring（MRM） under positive ion mode of electrospray ionization. Results The good linearity of four aflatoxins were achieved in the range of 0.5-50 ng/ml（r≥0.999 5）. The limits of detection were 0.02-0.04 μg/kg,and the limits of quantification were 0.07-0.13 μg/kg. The average recoveries of spiked samples were 75.6%-98.5% with the relative standard devitation of 3.9%-7.1%. Conclusion The method is simple,accurate, sensitive and applicable to the determination of aflatoxins B1,B2,G1 and G2in tea.

关 键 词：茶叶 黄曲霉毒素 高效液相色谱 串联质谱 

分 类 号：O657.6[理学—分析化学]