Molecularly imprinted polymer for pre-concentration of esculetin from tobacco followed by the UPLC analysis  

作　　者：RAZWAN SARDAR M. JIN Yan KONG Guang Hui LI Hai Fang LIN Jin-Ming 

机构地区：[1]Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University [2]Nova Med Pharmaceuticals Pvt. Ltd. [3]Yunnan Academy of Tobacco Agricultural Science

出　　处：《Science China Chemistry》2014年第12期1751-1759,1-2,共9页中国科学（化学英文版）

基　　金：supported by the Yunnan Green Tobacco Production Research(2011YN01);the China Equipment and Education Resources System(CERS-1-75)

摘　　要：Molecularly imprinted polymers(MIPs)for solid-phase extraction and pre-concentration of esculetin have been successfully prepared by the bulk polymerization method using esculetin as a template molecule.Polymers of varying composition were prepared using different monomers(4-vinylpyridine,methacrylic acid,and acrylamide),ethylene glycol dimethacrylate as the cross-linker,2,2-azobis(2-methylpropinitrile)as the initiator,and different porogen solvents(ethanol,acetone/methanol,and acetonitrile).The best polymer was obtained when 4-vinylpyridine was used as the monomer and acetone/methanol(3:2)as the porogen solvent,whereas the template:-monomer:-cross-linker ratio was 1:4:20.The imprinting factor of the selected MIPs for esculetin was 3.77.The polymers were evaluated according to their selective recognition properties for esculetin and structurally-related compounds(esculin,scopoletin,coumarin,and 7-methoxycoumarin).Chemical and morphological characterizations of the polymers were investigated by FTIR and scanning electron microscope,which confirmed a high degree of polymerization.Surface area,pore volume,and pore size of the polymer were investigated by Brunauer-Emmett-Teller analysis.MIPs were also successfully used as solid-phase adsorbent materials for the extraction of esculetin from tobacco leaves.Esculetin contents in dried tobacco leaves were found to be(9.27±0.17)μg g-1.Molecularly imprinted polymers（MIPs）for solid-phase extraction and pre-concentration of esculetin have been successfully prepared by the bulk polymerization method using esculetin as a template molecule.Polymers of varying composition were prepared using different monomers（4-vinylpyridine,methacrylic acid,and acrylamide）,ethylene glycol dimethacrylate as the cross-linker,2,2-azobis（2-methylpropinitrile）as the initiator,and different porogen solvents（ethanol,acetone/methanol,and acetonitrile）.The best polymer was obtained when 4-vinylpyridine was used as the monomer and acetone/methanol（3：2）as the porogen solvent,whereas the template：-monomer：-cross-linker ratio was 1：4：20.The imprinting factor of the selected MIPs for esculetin was 3.77.The polymers were evaluated according to their selective recognition properties for esculetin and structurally-related compounds（esculin,scopoletin,coumarin,and 7-methoxycoumarin）.Chemical and morphological characterizations of the polymers were investigated by FTIR and scanning electron microscope,which confirmed a high degree of polymerization.Surface area,pore volume,and pore size of the polymer were investigated by Brunauer-Emmett-Teller analysis.MIPs were also successfully used as solid-phase adsorbent materials for the extraction of esculetin from tobacco leaves.Esculetin contents in dried tobacco leaves were found to be（9.27±0.17）μg g-1.

关 键 词：molecularly imprinted polymer ESCULETIN TOBACCO UPLC 

分 类 号：O631.3[理学—高分子化学]