形觉剥夺性近视豚鼠视网膜形态学观察及TGF-β_2表达  

Structure changes of retina and expression of TGF- β_2 in the retina in form deprivation myopia of guinea pigs

作　　者：周凌霄[1] 王理论[2] 张林[3] 

机构地区：[1]西安医学院第一附属医院眼科,中国陕西省西安市710077 [2]延安大学附属医院眼科,中国陕西省延安市716000 [3]西安交通大学第一附属医院眼科,中国陕西省西安市710061

出　　处：《国际眼科杂志》2014年第11期1950-1952,共3页International Eye Science

摘　　要：目的:观察形觉剥夺性近视豚鼠视网膜组织学改变和TGF-β2在视网膜中的表达,为阐明TGF-β2在形觉剥夺性近视中的重要作用提供实验依据。方法:用头套形觉剥夺右眼的方法诱导近视动物模型。实验前后检测豚鼠双眼屈光状态及眼轴长度。HE染色后观察后极部视网膜形态学改变,通过免疫组化对TGF-β2在视网膜内表达进行定性及半定量测定。结果:实验组视网膜变薄,且内核层的胞核层数较对照眼少,视网膜外核层细胞核变小、变圆,排列不均,视细胞层外节内节排列紊乱。免疫组化检查示实验组视网膜TGF-β2表达明显下调。结论:头套遮盖效果明显,是一种诱导形觉剥夺性近视简便、稳定的方法。形觉剥夺性近视眼的视网膜产生了一系列的退行性改变。TGF-β2可能是最终导致形觉剥夺性近视形成的重要因素之一。AIM： To observe the structure changes of retina and determine the expression of TGF-β2 in retina model. At the same time to clarify the function of TGF-β2 in form deprivation myopia model. METHODS： A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia. Before and after experiment, refraction was measured using retinoscope, and ocular axial was determined by A-scan ultrasonography. Morphologic alternations of the retina were observed by light microsope. The expression of TGF 152 in the retina was determined by immunohistochemistry. RESULTS：Retina of myopic eyes became thinner, and inner nuclear layer of retina had few layers, nuclear of outer nuclear layer became smaller and circular, at the same time, they arranged in disorder. The immunohistochemisty results showed that expression of TGF-152 in model eyes was lower than that in control eyes （P〈0.05）. CONCLUSION： Covering eyes with facemasks is effective, simple and convenient in making myopicmodel. The retina of the model eye appeares degeneration. TGF-132 plays an important role in the course of development in form deprivation myopia.

关 键 词：形觉剥夺性近视 视网膜 TGF-Β2 

分 类 号：R778.11[医药卫生—眼科]