Caspase-14在恶性黑素瘤中的表达及意义  

Human caspase-14 expression in malignant melanoma and its significance

作　　者：王馨苑[1] 张霁[2] 承尧 杜吉佩[2] 陈德高[1] 杨志梅[1] 王玉芳[1] 黄宁[1] 

机构地区：[1]四川大学华西基础医学与法医学院病理生理学教研室,四川成都610041 [2]四川大学华西基础医学与法医学院法医物证学教研室,四川成都610041

出　　处：《细胞与分子免疫学杂志》2014年第11期1180-1183,1189,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金青年项目(81201560);四川大学校青年基金(2012SCU11900-7);四川大学青年教师科研启动基金(2012SCU11020)

摘　　要：目的明确caspase-14在恶性黑素瘤细胞和组织中表达水平及其对肿瘤细胞耐药的影响。方法运用反转录PCR和Western blot技术检测人黑素细胞和恶性黑素瘤细胞内caspase-14的表达。恶性黑素瘤和皮内痣组织中caspase-14表达采用原位杂交和免疫组织化学方法检测。结果 Caspase-14在恶性黑素瘤细胞和黑素细胞中均有表达。T细胞识别的黑素瘤相关抗原1(MART-1)阳性肿瘤细胞中caspase-14表达高于MART-1阴性细胞。Caspase-14表达水平低的肿瘤细胞对化疗药物更敏感(P<0.01)。在临床样本中,caspase-14在皮内痣中阳性率为70%,恶性黑素瘤组织中阳性率为97%,差别有统计学意义(P<0.05)。结论 Caspase-14在恶性黑素瘤细胞和组织中均有表达。Caspase-14表达水平高的恶性黑素瘤细胞对化疗药物喜树碱和顺铂,以及放射治疗更加抵抗。Objective To detect the caspase-14 expression in malignant melanoma cells and tumor tissues and its effect on tumor resistance to drug. Methods The mRNA and protein level of caspase-14 in 4 melanoma cell lines（ A375,A875,M14,and SK-Mel-1） and the melanocytes,was detected by reverse transcription PCR（ RT-PCR） and Western blotting.Caspase-14 expression in 34 malignant melanoma tumor tissues and 10 dermal nevus tissues was determined by in situ hybridization and immunohistochemistry. Results Caspase-14 expression was seen in melanoma cells and melanocytes. It was higher in melanoma-associated antigen 1 recognized by T cells（ MART-1） positive cells than in MART-1 negative cells. The cells expressing the lower caspase-14 were more sensitive to the treatment with either chemotherapy drugs camptothecin and cisplatin or radiotherapy than the ones expressing the higher caspase-14（ P〈0. 01）. Caspase-14 expression was observed in 70% dermal nevus,as well as 97% in malignant melanoma tissues,and the difference between them was statistically significant（ P〈0. 05）. Conclusion Caspase-14 is expressed in tissues and cells of malignant melanoma. Our data indicated that the expression level of caspase-14 affected the drug sensitivity of melanoma.

关 键 词：恶性黑素细胞瘤 caspase-14 化学疗法 

分 类 号：R739.5[医药卫生—肿瘤] R73-361[医药卫生—临床医学]