Annexin V-FITC/PI双标记与Hoechst33342/PI双标记流式细胞术检测细胞凋亡的比较  

Comparison between annexin V-FITC/PI and Hoechst33342/PI double stainings in the detection of apoptosis by flow cytometry

作　　者：张伟[1] 梁智辉[2] 

机构地区：[1]武汉科技大学医学院基础部,湖北武汉430065 [2]华中科技大学同济医学院细胞工程中心,湖北武汉430030

出　　处：《细胞与分子免疫学杂志》2014年第11期1209-1212,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30972697)

摘　　要：目的比较annexin V-FITC/PI双标记与Hoechst33342/PI双标记流式细胞术检测细胞凋亡的优缺点。方法用DEX诱导胸腺细胞凋亡和H2O2诱导K562细胞系细胞凋亡,应用Hoechst33342/PI和annexin V-FITC/PI双色标记流式细胞术检测体系检测细胞凋亡情况。结果 Annexin V-FITC/PI双标记法能准确地反映2种模型中的细胞凋亡情况,与其检测原理一致;而Hoechst33342/PI双标记法虽能准确反映DEX诱导胸腺细胞的凋亡情况,但在检测H2O2诱导的K562细胞凋亡时,其早期凋亡细胞的分布与其检测原理明显不符。结论与annexin-V-FITC/PI双标记法相比,Hoechst33342/PI双标记法有一定的局限性。Objective To evaluate the merits and demerits of annexin V-FITC/PI and Hoechst33342/PI double stainings in the detection of apoptosis by flow cytometry. Methods Hoechst33342 /PI and annexin V-FITC /PI double stainings were performed to detect the apoptosis of thymocytes induced by DEX and K562 cells induced by H2O2. Results The anenxin V-FITC /PI double staining was able to detect the cell apoptosis in both DEX-induced thymocytes and H2O2-induced K562 cells as predicted,and the cell plotting was consistent with the principle of the method. Hoechest33342 /PI double staining was able to detect cell apoptosis in the DEX-induced thymocytes,but this method showed a contradictory result in the early stage of H2O2-induced K562 cell apoptosis,and the cell plotting was in conflict with the principle of the method. Conclusion Compared with annexin V-FITC /PI double staining,the method of Hoechst33342 /PI double staining in the detection of apoptosis by flow cytometry has some limitations.

关 键 词：凋亡 ANNEXIN V-FITC/PI Hoechst33342/PI 流式细胞术 

分 类 号：Q2-33[生物学—细胞生物学] Q243