原发性肝癌组织中B7-H3表达与浸润的CD8^+ T细胞数负相关  

A negative correlation between B7-H3 expression and the number of CD8^+ T cell infiltration in primary hepatocellular carcinoma tissues

作　　者：刘同慎[1] 霍玉青 李广云[3] 于国华[4] 栾希英[1] 

机构地区：[1]滨州医学院基础学院,山东烟台264003 [2]滨州医学院附属医院,山东滨州256603 [3]山东省千佛山医院,山东济南250014 [4]烟台市毓璜顶医院,山东烟台264000

出　　处：《细胞与分子免疫学杂志》2014年第12期1291-1294,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省自然科学基金(ZR2010HL048);山东省医药卫生发展计划(2013WS0132);滨州医学院科技计划(BY2012KJ42)

摘　　要：目的探讨原发性肝癌组织中B7-H3的表达与CD8+T细胞浸润之间的关系。方法采用免疫组织化学技术分析原发性肝癌组织和正常肝组织中B7-H3的表达、CD8+T细胞的浸润数量,对B7-H3的表达与CD8+T细胞浸润数量的相关性进行统计学分析。结果 63例肝癌患者中B7-H3高表达26例(41.3%),低表达37例(58.7%),总阳性率为90.5%,其中正常肝组织基本不表达B7-H3。B7-H3高表达组CD8+T细胞浸润数量明显低于B7-H3低表达组,CD8+T细胞浸润数量与肝癌组织中B7-H3的表达呈负相关。肝癌组织中B7-H3的表达和CD8+T细胞数量在肝癌患者的临床病理分期、是否有局部淋巴结转移以及远隔器官转移等方面具有显著性差异。结论原发性肝癌组织中高表达B7-H3,与肿瘤组织中CD8+T细胞浸润呈负相关。Objective To investigate the relationship between B7-H3 expression and the number of CD8＋ T cell infiltration in primary hepatocellular carcinoma（HCC） tissues. Methods The level of B7-H3 expression and the number of CD8＋T cell infiltration in primary HCC were determined using immunohistochemical staining,and then the correlation between them was analyzed statistically. Results The immunohistochemical staining showed that the high expression of B7-H3 was found in 26cases（41. 3%）,and the low expression in 37 cases（58. 7%） of the 63 primary HCC. Fifty-seven cases（90. 5%） were positive for B7-H3 expression in HCC tissues,and normal hepatocellular tissues hardly expressed B7-H3 molecules. The number of infiltrating CD8＋T cells in B7-H3 high expression group was less than that in B7-H3 low expression group. B7-H3 expression was negatively correlated with the number of infiltrating CD8＋T cells in HCC tissues. Moreover,both B7-H3 expression and CD8＋T cell infiltration were related with clinical stage,local lymph node metastasis and distant metastasis in HCC patients. Conclusion B7-H3 molecules were highly expressed and negatively correlated with CD8＋T cell infiltration in primary HCC tissues.

关 键 词：原发性肝癌 B7-H3 CD8+T细胞 肿瘤免疫 

分 类 号：R735.7[医药卫生—肿瘤] R392-33[医药卫生—临床医学]