nm23-H1转染对肺癌细胞钙调素和基质金属蛋白酶表达的影响  

Influences of transfection of nm23-H1 on expression of E-cadherin and matrix metalloproterinases in lung cancer cell

作　　者：郑海霞[1] 申东兰[2] 彭安[2] 何艳玲[2] 

机构地区：[1]北京大学深圳医院体检科,广东深圳518036 [2]北京大学深圳医院肿瘤内科,广东深圳518036

出　　处：《现代肿瘤医学》2014年第12期2771-2773,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30070333)

摘　　要：目的:探讨nm23-H1转染对肺癌细胞钙调素和基质金属蛋白酶表达的影响。方法:利用半定量RT-PCR技术检测转染前后E-cadherin、MMP-9基因的mRNA表达;利用流式细胞仪技术检测转染前后Ecadherin、MMP-9基因的蛋白表达。结果:转染后细胞株E-cadherin mRNA与蛋白表达上调,转染后细胞株MMP-9 mRNA与蛋白表达下调。结论:nm23-H1基因对肺癌细胞株L9981 E-cadherin mRNA与蛋白表达具有正调控作用,对MMP-9 mRNA与蛋白表达具有负调控作用。Objective： To study influences of the transfection of nm23- H1 gene on the expression of E-cadherin and matrix metalloproterinases in lung cancer cell. Methods： Semi- RT- PCR was used to detect the mRNA expression of E- cadherin and MMP- 9 in transfected and non- transfected cell lines,Flow cytometry was used to detect the protein expression of E- cadherin and MMP- 9 in transfected and non- transfected cell lines. Results： The expression of mRNA and protein of E- cadherin of transfected cell line was stronger than that of non- transfected cell line. The expression of mRNA and protein of MMP- 9 of transfected cell line was weaker than that of non- transfected cell line. Conclusion： nm23- H1 can up- regulate expression of E- cadherin,down- regulate expression of MMP- 9 and thus inhibit the metastasis of lung cancer cell.

关 键 词：NM23-H1 肺癌细胞 钙调素 MMP-9 肿瘤转移 

分 类 号：R730.23[医药卫生—肿瘤] R734.2[医药卫生—临床医学]