miR-200a在肝癌干细胞中的差异表达及其作用  

The function and differential expression of miR-200a in liver cancer stem cells

作　　者：汪建林[1] 杨西胜 王兴[1] 戴斌[1] 阮柏[1] 高远[1] 李海民[1] 

机构地区：[1]第四军医大学西京医院肝胆外科,陕西西安710032

出　　处：《现代肿瘤医学》2014年第12期2785-2788,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172061)

摘　　要：目的:分离肝癌细胞系MHCC97-H中肝癌干细胞并分析miR-200a在肝癌干细胞和非肝癌干细胞亚群中的表达差异,探讨miR-200a的表达水平与肝癌干细胞之间的关系。方法:利用流式细胞荧光激活分选法从MHCC97-H中分选出肝癌干细胞(liver cancer stem cells,LCSCs)和非肝癌干细胞(non-liver cancer stem cells,non-LCSCs)两个亚群;采用real-time PCR检测miR-200a在两个亚群中的表达差异;在MHCC97-H中瞬时转染miR-200a-mimic,检测LCSCs亚群比例的变化。结果:LCSCs亚群占总体细胞的百分比为3.56%;LCSCs亚群细胞中miR-200a的表达明显低于non-LCSCs亚群(P<0.05);上调miR-200a后LCSCs亚群细胞比例为1.24%。结论:miR-200a在LCSCs亚群细胞中明显低表达,上调miR-200a后LCSCs亚群细胞比例明显降低,提示miR-200a能够调控肝癌干细胞的比例。通过调控miR-200a的表达,可以降低LCSCs亚群细胞比例,从而为肝癌的治疗提供新的思路。Objective： To isolate and enrich liver cancer stem cells（ LCSCs） from MHCC97- H cells and investigate the expression of miR- 200 a in LCSCs and non- LCSCs. Methods： LCSCs and non- LCSCs were isolated and enriched from MHCC97- H cells by using flow cytometry. The expression of miR- 200 a in LCSCs and non- LCSCs were measured by real- time PCR. MHCC97- H cells transfected with miR- 200 a mimic or miR- NC,after transfected,detected the percentage of LCSCs by flow cytometry. Results： LCSCs were found to account for 3. 56%. The expression of miR- 200 a in LCSCs was significantly lower than non- LCSCs（ P 0. 05）. After transfected miR- 200a- mimic in MHCC97- H,the percentage of LCSCs was 1. 24%. Conclusion： The significantly down- regulated expression of miR- 200 a in LCSCs,and after transfected miR- 200a- mimic in MHCC97- H,the percentage of LCSCs was reduced indicated that miR- 200 a may be involved in the regulation of the percentage of LCSCs. We could reduce the percentage of LCSCs through regulation the expression of miR- 200 a,which will offer a novel therapeutic strategy for the treatment of HCC.

关 键 词：miR-200a 肝癌 肝癌干细胞 

分 类 号：R730.23[医药卫生—肿瘤] R735.7[医药卫生—临床医学]