MiRNA-21抑制物对人肺腺癌PC9/GR细胞吉非替尼耐药性的影响  

Effect of microRNA-21 inhibitors on the gefitinib resistant of the human lung adenocarcinoma PC9/GR cells

作　　者：李明涛[1] 王小平[1] 

机构地区：[1]第四军医大学唐都医院胸外科,陕西西安710038

出　　处：《现代肿瘤医学》2014年第12期2789-2792,共4页Journal of Modern Oncology

摘　　要：目的:观察miR-21抑制物对人肺腺癌PC9/GR细胞株增殖、凋亡及迁移能力的影响,探讨miR-21抑制物在PC9/GR细胞吉非替尼耐药的生物学行为中的作用。方法:实验分为miR-21 inhibitor组、阴性对照组和正常细胞对照组3组。MiR-21 inhibitor组转染miR-21抑制物抑制其细胞内miR-21表达,阴性对照组转染microRNA inhibitor NC。加入吉非替尼处理后,采用MTT比色实验测定3组细胞第1、2、3、4、5天的吸光度(OD值),绘制生长曲线。采用流式细胞仪检测细胞凋亡,Transwell法检测细胞迁移。结果:两对照组间细胞的增殖速度无明显差异(P>0.05),与对照组相比,实验组细胞增殖速度明显减慢(P<0.05)。实验组细胞凋亡率为(40.06±2.32)%,明显增高(P<0.05);阴性对照组[(7.39±0.79)%]和空白对照组[(6.96±0.68)%]之间细胞凋亡无明显差异(P>0.05)。实验组细胞迁移力受到明显抑制(P<0.05)。空白对照组与阴性对照组细胞迁移力相比无明显差异(P>0.05)。结论:在PC9/GR细胞中,下调miRNA-21的表达后,PC9/GR细胞对GR的敏感性提高,凋亡程度增强,部分逆转了肺癌PC9/GR细胞对GR的耐药性。为miRNA-21与化疗联合治疗肺癌提供了实验依据。Objective： To investigate the effect of microRNA- 21 inhibitors on the biological behaviour of PC9 /GR cells gefitinib resistant by observing the role of microRNA- 21 inhibitors in cell proliferation,apoptosis and migration of human lung adenocarcinoma PC9 / GR cells. Methods： The experiment contained three groups： MiR- 21 inhibitor group（ transfected with hsa- miR- 155 inhibitors）,control group（ transfected with microRNA inhibitor NC） and blank group. After added gefitinib,detected the MTT value（ OD） at 1d,2d,3d,4d,and 5d,then showed the cell growth curve. The apoptosis was measured by flow cytometry. Cell number of migration were measured by Transwell. Results：Through comparison between the three groups,there ＇s no obvious effect between the control group and blank group（ P 0. 05）,and the experimental group showed lower cell proliferation of efficiency than the control group（ P 0. 05）.The apoptosis ratio of the experimental group（ 40. 06 ± 2. 32） % was much higher than that of the control group and blank group（ P 0. 05）,there ＇s no difference between control group（ 7. 39 ± 0. 79） % and blank group（ 6. 96 ±0. 68） %（ P 0. 05）. The migration of the experimental group cells were significantly inhibited（ P 0. 05）. Conclusion： In PC9 / GR cells,downregulated expression of miR- 21 improved the sensitivity of PC9 / GR cells to GR,enhanced the apoptosis degree,partly reversed the drug resistance in lung cancer PC9 / GR cells to GR. To provide the possible experimental basis for miRNA- 21 combined with chemotherapy in the treatment of lung cancer.

关 键 词：PC9/GR MIR-21 增殖 凋亡 迁移 

分 类 号：R73-36[医药卫生—肿瘤] R734.2[医药卫生—临床医学]