转录因子FOXC1、基质金属蛋白酶7在不同乳腺癌分子亚型中的表达及与临床病理的相关性分析  

Expressions of FOXC1 and MMP-7 in molecular subtypes of breast cancer and their association with clinicopathological characteristics

作　　者：曹培龙[1] 王凯[1] 王春宝[1] 李晓峰[1] 杨喆[1] 王鸿雁[1] 

机构地区：[1]西安交通大学医学院第一附属医院病理科

出　　处：《浙江大学学报（医学版）》2014年第4期406-412,共7页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的：观察转录因子FOXC1、基质金属蛋白酶7（MMP-7）在乳腺癌各分子亚型中的表达情况，探讨FOXC1、MMP-7对乳腺癌分子亚型的诊断及预后价值。方法：105例乳腺癌患者根据免疫组织化学检测ER、PR、HER2、CK5／6、CK14、EGFR结果分为四型：腔型、HER2阳性型、基底细胞样型（BLs型）及正常乳腺样型（NBLs型）。观察各乳腺癌分子亚型的临床特征及与FOXC1、MMP-7的关系。结果：在105例乳腺癌患者中腔型、HER2阳性型、BLs型和NBLs型乳腺癌所占比例分别为52．4％（55／105）、16．2％（17／105）、17．1％（18／105）、14．3％（15／105）。腔型和（或）NBLs型乳腺癌患者5年生存率明显高于HER2阳性型和（或）BLs型患者（10g—rank值为22．161，P〈0．01）。FOXC1阳性表达共28例，阳性表达率为26．7％（28／105），FOXC1表达与肿瘤组织学分级、肿瘤大小、远处转移及患者5年生存率有关，而且FOXC1在BLs型乳腺癌中阳性表达率显著高于其他分子亚型乳腺癌（χ^2=30．108，P〈0．01）。MMP-7阳性表达共71例，阳性表达率为67．6％（71／105），MMP-7与患者同侧腋窝淋巴结转移、远处转移及患者5年生存率有关。MMP-7在BLs型乳腺癌中阳性表达率也高于其他分子亚型乳腺癌（χ^2=11．328，P〈0．05）。FOXC1与MMP-7的表达呈正相关（r=0．325，P〈0．01）。结论：腔型和NBLs型乳腺癌患者预后较好，HER2阳性型和BLs型乳腺癌预后较差。FOXC1可能作为BLs型乳腺癌的特异性潜在分子标志物和潜在治疗靶点。MMP-7可能作为判断乳腺癌侵袭性、恶性程度及评估预后的有用指标。FOXC1与MMP-7在分子机制上可能存在一定的联系。Objective： To investigate the expressions of FOXC1 and matrix metalloproteinase 7 （MMP-7） in different molecular subtypes of breast cancer and their association with clinicopathological characteristics of the disease. Methods： Based on immunohistochemical results of ER, PR, HER2, CKS/6, CK14 and EGFR, 105 breast cancer patients were classified as 4 subtypes： luminal, HER2 positive, basal-like subtype （BLs） and normal breast-like subtype （NBLs）. The association of FOXC1 and MMP-7 expressions with clinicopathological parameters in different molecular subtypes was analyzed. Results： Out of 105 patients with breast cancer, the subtypes of luminal, HER2 positive, BLs and NBLs accounted for 52.4% （55/105）, 16.2% （17/105）, 17. 1 % （18/105） and 14.3% （15/105）, respectively. Patients with luminal and/or NBLs subtypes had significantly higher 5 -year survival rate than those with HER2 positive and!or BLs did （log-rank = 22. 161, P 〈 0.01）. The overall positive expression rate of FOXC1 was 26. 7% （28/105） and the expression was higher in BLs patients than that in other subgroups （χ^2 = 30. 108, P 〈 0.01）. The expression of FOXC1 was correlated with histological grade, tumor size, distant metastasis and 5- year survival rate of breast cancer. The overall positive expression rate of MMP-7 was 67.6% （71/105） and the expression of MMP-7 was also higher in BLs patients than that in other molecular subtypes （χ^2 = 11. 328, P 〈 O. 05）. The positive expression of MMP-7 was correlated with metastasis in ipsilateral axillary lymph nodes, distant metastasis and 5-year survival rate of the patients. FOXC1 was correlated with MMP-7 （r=0.325,P〈0.01）. Conclusion： Patients with luminal and/or NBLs breast cancer have more favorable prognosis than those with HER2 positive and/or BLs subtypes. The expressions of FOXC1 and MMP-7 are closely correlated with the clinicopathological features of breast cancer patients.

关 键 词：乳腺肿瘤/病理学 乳腺肿瘤/诊断 基质金属蛋白酶7/代谢 转录因 子/代谢 基因表达 预后 免疫组织化学 随访研究 

分 类 号：R737.9[医药卫生—肿瘤]