富血小板血浆及脐带间充质干细胞修复软骨损伤  

Platelet-rich plasma plus human umbilical cord mesenchymal stem cells for cartilage repair

作　　者：徐静[1] 王黎明[1] 周立冬[2] 吴美[2] 崔辉[2] 赵璟[2] 曾独娟[2] 张仲文[3] 刘爱兵[2] 

机构地区：[1]安徽医科大学武警总医院临床学院,安徽省合肥市230032 [2]武警总医院医学实验中心,北京市100071 [3]武警总医院骨四科,北京市100039

出　　处：《中国组织工程研究》2014年第41期6633-6638,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究者直接将富血小板血浆作为支架材料与骨髓基质干细胞、软骨细胞等复合后体外培养发现软骨细胞在富血小板血浆三维支架呈现增殖生长,骨髓基质干细胞在增殖的同时有向软骨细胞分化的倾向。目的:观察富血小板血浆和人脐带间充质干细胞对受损软骨修复的影响。方法:正常健康新西兰大白兔40只,制备兔软骨损伤模型。2次离心法制备富血小板血浆,制备3代人脐带间充质干细胞。随机将动物分为4组,造模后生理盐水组关节腔一次性注入生理盐水0.5 mL;富血小板血浆组注入12.5%富血小板血浆0.5 mL;人脐带间充质干细胞组注入1×107人脐带间充质干细胞0.5 mL;富血小板血浆(12.5%)联合人脐带间充质干细胞(1×107)组注入两种物质0.5 mL。造模后第12周,大体观察软骨损伤修复情况;苏木精-伊红染色光镜下观察损伤部位细胞修复情况;根据O’Driscoll组织学评分标准对造模后第12周切片进行组织学评分。结果与结论:软骨损伤后大体观察、苏木精-伊红染色组织学观察及组织学评分均显示富血小板血浆组、人脐带间充质干细胞组、富血小板血浆联合人脐带间充质干细胞组对软骨损伤的修复效果优于生理盐水组,差异有显著性意义(P<0.05),富血小板血浆组、富血小板血浆联合人脐带间充质干细胞组的修复效果优于人脐带间充质干细胞组,差异有显著性意义(P<0.05)。结果说明富血小板血浆、人脐带间充质干细胞均能促进软骨损伤的修复,而且富血小板血浆、富血小板血浆联合人脐带间充质干细胞较单独应用人脐带间充质干细胞效果好。BACKGROUND：Chondrocytes co-cultured with bone marrow stromal stem cells on the scaffold of platelet-rich plasma are found to proliferate, and besides proliferative growth, bone marrow stromal cells exhibit a tendency of differentiating into chondrocytes. OBJECTIVE：To study the effect of platelet-rich plasma and human umbilical cord mesenchymal stem cells （hUCMSCs） on cartilage repair. METHODS：Forty healthy New Zealand white rabbits were selected to establish models of cartilage defects, and then randomly divided into normal saline group, platelet-rich plasma group, hUCMSCs group and combination group. Platelet-rich plasma was prepared by using double centrifugations to prepare passage 3 hUCMSCs. After modeling, intra-articular injection of normal saline （0.5 mL）, 12.5%platelet-rich plasma （0.5 mL）, 1×10^7 hUCMSCs （0.5 mL）, 12.5%platelet-rich plasma＋1×10^7 hUCMSCs （total y 0.5 mL） was done in corresponding groups, respectively. After 12 weeks of modeling, the injured cartilage was grossly observed, and hematoxylin-eosin staining was used to observe cartilage repair under light microscope;according to the O＆#39;Driscol histologic standard, histological examination was performed. RESULTS AND CONCLUSION：The repair effect in the normal saline group was significantly better that in the platelet-rich plasma group, hUCMSCs group, combination group （P〈0.05）, while the platelet-rich plasma group and combination group also exhibit better outcomes than the hUCMSCs group （P〈0.05）. These findings indicate that both platelet-rich plasma and hUCMSCs can promote cartilage repair;moreover, platelet-rich plasma with or without hUCMSCs is superior to hUCMSCs alone in the cartilage repair.

关 键 词：干细胞 脐带脐血干细胞 富血小板血浆 人脐带间充质干细胞 关节软骨 软骨损伤 骨关节炎 

分 类 号：R394.2[医药卫生—医学遗传学]