口服肉苁蓉联合骨髓间充质干细胞移植治疗大鼠脊髓损伤  

Cistanche deserticola plus bone marrow mesenchymal stem cell transplantation for treating spinal cord injury in rats

作　　者：兰静[1] 闫金玉[1] 夏润福[1] 李剑锋[1] 

机构地区：[1]内蒙古医科大学第二附属医院康复科,内蒙古自治区呼和浩特市010030

出　　处：《中国组织工程研究》2014年第41期6639-6644,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究表明骨髓间充质干细胞在特定的条件下可分化成为神经细胞,移植后可重建神经系统功能,改善患者功能障碍,肉苁蓉总苷也对损伤的神经细胞有保护作用,将二者联合应用的报道较少。目的:探讨口服肉苁蓉联合骨髓间充质干细胞移植修复大鼠脊髓损伤的效果。方法:55只成年Wistar大鼠制备脊髓损伤模型,随机分为4组,对照组每天20 mL/kg生理盐水灌胃;口服肉苁蓉组每天20 mL/kg肉苁蓉条件浓缩液灌胃;骨髓间充质干细胞移植组造模后即刻移植骨髓间充质干细胞悬液(1×108 L-1)10μL,每天20 mL/kg生理盐水灌胃;口服肉苁蓉+骨髓间充质干细胞移植组造模后即刻移植骨髓间充质干细胞悬液(1×108 L-1)10μL,每天20 mL/kg肉苁蓉条件浓缩液灌胃,各组连续灌胃30 d。结果与结论:治疗30 d后免疫组化法检测口服肉苁蓉+骨髓间充质干细胞移植组脊髓组织中Nestin阳性表达明显多于其他组;治疗12周后,口服肉苁蓉+骨髓间充质干细胞移植组BBB评分明显高于其他组,差异有显著性意义(P<0.05),感觉诱发电位和运动诱发电位潜伏期较其他组有明显改善(P<0.05)。结果表明口服肉苁蓉联合骨髓间充质干细胞移植可以明显改善脊髓损伤大鼠的运动功能及神经电生理功能;口服肉苁蓉可以有效促进移植的骨髓间充质干细胞在宿主内存活。BACKGROUND：Studies have shown that bone marrow mesenchymal stem cells under certain conditions can differentiate into nerve cells. Bone marrow mesenchymal stem celltransplantation can rebuild nervous system function and improve functional disorders in patients. Glycosides of cistanche also have a protective effect against nerve cellinjury. Their combination has been less reported. OBJECTIVE：To observe the effectiveness of Cistanche deserticola and bone marrow mesenchymal stem celltransplantation on spinal cord injury in rats. METHODS：Fifty adult Wistar rats with spinal cord injury were randomly divided into four group：a Cistanche deserticola group （intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day）, a celltransplantation group （10μL of 1×10^8/L bone marrow mesenchymal stem cellsuspension）, a combination group （10μL of 1×10^8/L bone marrow mesenchymal stem cellsuspension＋intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day） and a control group （intragastric administration of 20 mL/kg normal saline per day）. The intragastric administration lasted for 30 days in each group. RESULTS AND CONCLUSION：After 30 days of treatment, the expression of Nestin was significantly higher in the combination group than the other groups. After 12 weeks, Basso, Beattie, and Bresnahan scores was significantly higher in the combination group than the other groups （P〈0.05）;somatosensory and motor evoked potential latencies were also improve significantly in the combination group compared with the other groups （P〈0.05）. These findings indicate that oral administration of Cistanche deserticola combined with bone marrow mesenchymal stem cells can significantly improve the motor and neurophysiological function of spinal cord injury rats. Cistanche deserticola can improve the survival of transplanted bone marrow mesenchymal stem cells in rats with spinal cord injury.

关 键 词：干细胞 移植 肉苁蓉 骨髓间充质干细胞 脊髓损伤 WISTAR大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]