伽马射线对重组质粒(CArG)_6-hTERTp-SEA-GPI在人肿瘤A549细胞和成纤维细胞WI-38中的SEA表达的影响  

Effect of Gamma-Ray on the SEA Expression in A549 and WI-38 Cells Transfected with Plasmid of(CArG)_6-hTERTp-SEA-GPI

作　　者：吴朝妍[1] 张莹雯[1] 黄勇[2] 钟亚华[2] 於海军[2] 周福祥[2] 

机构地区：[1]武汉大学中南医院中西医结合科,湖北武汉430071 [2]武汉大学中南医院肿瘤放化疗科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2014年第6期821-824,共4页Medical Journal of Wuhan University

基　　金：教育部高等学校博士学科点专项科研基金资助项目(编号:20100141120014);国家自然科学基金青年项目(编号:81201755)

摘　　要：目的:观察γ-射线对重组质粒(CArG)6-hTERTp-SEA-GPI在人肿瘤A549细胞和人正常细胞WI-38中的SEA表达的影响。方法:重组质粒(CArG)6-hTERTp-SEA-GPI分别转染A549和WI-38细胞,转染后12h2Gyγ-射线照射肿瘤细胞和正常细胞,转染后24h后采用RT-PCR法检测SEA mRNA的表达,转染后48h采用Western blot法检测SEA蛋白的表达。结果:(CArG)6-hTERTp-SEA-GPI能够在肿瘤细胞A549细胞中表达SEA,正常细胞WI-38细胞中不能表达SEA;2Gyγ-射线可以诱导转染(CArG)6-hTERTp-SEA-GPI质粒的A549细胞SEA mRNA和蛋白的表达,表达量分别增加115.4%和24.2%。结论:重组质粒(CArG)6-hTERTp-SEAGPI能够在肿瘤细胞A549中选择性表达SEA,且2Gyγ-射线可以增强SEA的表达水平。Objective：To investigate the effect ofγ-Ray on the SEA expression in A549 and WI-38 cells transfected with plasmid of（CArG）6-hTERTp-SEA-GPI.Methods：A549cells and WI-38 cells were transfected with plasmid（CArG）6-hTERTp-SEA-GPI,and irradiated with 2Gyγ-ray after12 hours of transfection.SEA mRNA was detected by RT-PCR after 24 hours of transfection.SEA protein was measured with Western blot after 48 hours of transfection.Results：SEA mRNA and protein were expressed in A549 cells transfected with（CArG）6-hTERTp-SEA-GPI,but not in WI-38 cells transfected with（CArG）6-hTERTp-SEA-GPI.SEA mRNA and protein expression level increased by 115.4% and 24.2%in A549 cells transfected with（CArG）6-hTERTpSEA-GPI after 2Gyγ-ray radiation,but not in WI-38 cells.Conclusion：Plasmid（CArG）6-hTERTp-SEA-GPI can selectively express SEA mRNA and protein in human cancer cells A549,andγ-Ray radiation can enhance the expression level.

关 键 词：放射治疗 SEA HTERT启动子 CArG 肿瘤 

分 类 号：R730.58[医药卫生—肿瘤]