三硝基苯磺酸所致狭窄对大鼠结肠运动模式的影响  

Effects of TNBS-Induced Stricture on Colonic Motor Patterns

作　　者：杨自仙 王鹏宇[1] 陈继红[1] 张蛟[1] 邓宇晴[1] 俞媛洁[1] 郑也 张倩[1] 谭诗云[1] 罗和生[1] Jan D Huizinga 

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060 [2]麦克马斯持大学健康科学学院医学系

出　　处：《武汉大学学报（医学版）》2014年第6期835-838,866,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81170249);益普生腹泻基金(IDF)

摘　　要：目的:探讨三硝基苯磺酸(TNBS)所致狭窄对大鼠结肠运动模式的影响。方法:雄性SD大鼠32只随机分为TNBS造模组、TNBS+思密达组、思密达组及正常对照组,各组大鼠分别给予2%TNBS灌肠、2%TNBS灌肠+思密达灌胃、思密达灌胃及正常饲养。8d后处死并用时空图记录各组大鼠离体结肠的运动模式。结果:TNBS组远端结肠出现明显狭窄,长距离收缩运动(LDC)无法下传至远端结肠,狭窄部远端结肠出现独立于近端结肠运动模式的节律性运动模式,提示全结肠运动协调性丧失;狭窄部位以上近端结肠观察到LDC、节律性运动复合波(RPMCs)、微小波和分节运动等模式;TNBS+思密达组远端结肠未出现明显狭窄,全结肠协调性完整。结论:TNBS可致结肠神经源性损害、影响全结肠协调性,思密达可防止TNBS造成的结肠狭窄发挥神经保护作用,维护全结肠协调性。Objective：To explore the effects of TNBS-induced stricture on colonic motor patterns.Methods：Thirty-two SD rats were divided randomly and equally into four groups：A,B,C,and D according to the treatment TNBS,TNBS＋Smecta,Smecta,and normal saline（normal control）,respectively.All rats were killed 8days later and using spatiotemporal mapping to investigate the motor activities of the proximal colon.Results：Formation of strictures could be seen in the model of group A and no long distance contraction（LDC）develops.There were more complexes coming from the stricture,which were independent of the proximal colon;Motor patterns like rhythmic propulsive motor complexes,ripples,segmentation,proximal contractions and LDCs were observed in proximal colon;No obvious stricture appeared in group B and no changes in colonic motor patterns were found.Conclusion：The TNBS-induced stricture affects colonic motor patterns,but dioctahedral smectite can obviously prevent the TNBS-induced stricture,protectneurogenic components and maintain the coordination of movement of whole colon.

关 键 词：三硝基苯磺酸 狭窄 运动模式 思密达 时空图 

分 类 号：R574.62[医药卫生—消化系统]