MiR-203通过SNAI2的靶向作用对胃癌细胞SGC7901侵袭和凋亡的影响  

Effects of MiR-203 on Invasion and Apoptosis of SGC7901 Gastric Cancer Cell Through Targeting SNAI2

作　　者：胡俊华[1] 王琦[1] 杨艳果[1] 卢光新[1] 

机构地区：[1]湖北医药学院附属人民医院消化内科,湖北十堰442000

出　　处：《武汉大学学报（医学版）》2014年第6期857-861,888,共6页Medical Journal of Wuhan University

摘　　要：目的:研究miR-203通过SNAI2对胃癌细胞SGC7901侵袭和凋亡的影响。方法:1脂质体转染将miR-203mimics转入胃癌细胞SGC7901,Real-time PCR检测转染效果,Transwell实验和流式细胞术检测细胞侵袭和凋亡情况。2Real-time PCR和Western blot检测转染miR-203后在胃癌细胞中SNAI2的表达水平。3siRNA干扰胃癌细胞SGC7901中SNAI2的水平,Western blot验证敲减效果,并检测敲减SNAI2后细胞侵袭和凋亡情况。4转染miR-203mimics后,脂质体转染SNAI2表达质粒,检测细胞侵袭和凋亡情况。结果:1脂质体介导的miR-203mimics转染SGC7901后,细胞侵袭能力减弱,凋亡增加。2在胃癌细胞中,miR-203与SNAI2表达负相关。3敲减SNAI2后,细胞侵袭能力减弱,凋亡增加。4在过表达miR-203的胃癌细胞中转染SNAI2后能使胃癌细胞侵袭能力恢复,凋亡减少。结论:miR-203能够通过靶向调控SNAI2而降低胃癌细胞SGC7901的侵袭能力并促进其凋亡。Objective：To investigate the effect of miRNA-375 on cell invasion and apoptosis in the human gastric cancer cell line SGC7901.Methods：The expression levels of miRNA-203 in the SGC7901 cells were determined by real-time PCR after transfection of the miRNA-203 mimic.Transwell cell invasion and flow cytometry were used to detect the invasion and apoptosis of SGC7901 cells.Then the expression levels of SNAI2 in gastric cancer cell lines were assayed after transfection of the miRNA-203 mimic by real-time PCR and Western blot.The expression of SNAI2 was determined by Western blot after knockdown by siRNA,and the invasion and apoptosis of SGC7901 cells after knockdown of SNAI2 was detected.Finally,SNAI2 after transfection of miR-203 mimic was expressed,and the invasion and apoptosis of SGC7901 cells was detect.Results：The invasive abilities of SGC7901 were decreased and apoptosis level was increased when miR-203 expression was upregulated.The expression level of SNAI2 and miR-203 was negatively correlated in SGC7901 gastric cancer cell.The invasive ability of SGC7901 were decreased and ap-optosis level was increased after knockdown of SNAI2.Overexperssion of SNAI2 can reverse the effect of miR-203 on invasive abilities and apoptosis level of SGC7901.Conclusion：MiR-203 can inhibit invasion and promote apoptosis of SGC7901 gastric cancer cell by targeting SNAI2.

关 键 词：胃癌 miR-203 SNAI2 侵袭 凋亡 

分 类 号：R745.2[医药卫生—神经病学与精神病学]