橘小实蝇蛋白饵剂中氨基酸的HPLC分析及生物测定  

HPLC Analysis and Biological Test of Amino Acids in Protein Bait for Bactrocera dorsalis(Hendel)

作　　者：韩英[1] 王波[1] 许晨[2] 许建中[2] 黄居昌[1] 季清娥[1] 杨建全[1] 陈家骅[1] 

机构地区：[1]福建农林大学益虫研究所,福州350002 [2]国家海洋局第三海洋研究所,厦门361005

出　　处：《农业生物技术学报》2014年第11期1447-1454,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金面上项目(No.11175046)

摘　　要：采用生物测定的方法比较Prima蛋白饵剂和自行研制蛋白饵剂的引诱效果。结果表明,两者对橘小实蝇(Bactrocera dorsalis(Hendel))引诱效果相当,但水解后的同种蛋白饵剂引诱效果高于水解前。采用柱前衍生高效液相色谱法(Acc Q·Tag法)测定以上两种蛋白饵剂中氨基酸的种类和含量,结果表明,这两种蛋白饵剂中均含有游离氨基酸17种,总氨基酸18种。其中Prima蛋白饵剂总氨基酸含量为52.847 6mg/m L,游离氨基酸含量为5.736 4 mg/m L,多肽含量为4.711 12 mg/m L;自行研制的橘小实蝇蛋白饵剂总氨基酸含量为65.624 3 mg/m L,游离氨基酸含量为6.301 0 mg/m L,多肽含量为59.323 3 mg/m L。通过比较两者氨基酸的差别,为蛋白饵剂的工业化生产质量监控提供方法依据。This article mainly compares the similarities and differences between Prima produced by Malaysia Pupuk Alam company and protein bait for Bactrocera dorsalis （Hendel） developed by our research institute. The biological test was used to compare attractive effects, and High Performance Liquid Chromatography with Precolumn Derivatization （AccQ·Tag） was used to analyse the kinds and contents of amino acids. The results showed the attractive effects of Prima and protein bait were similar, and the two protein baits all contained 17 kinds of free amino acids and 18 total amino acids. Prima contained 52.847 6 mg/mL of total amino acids, 5.736 4 mg/mL of free amino acid, and 47.111 2 mg/mL of peptides. The protein bait was consisted of 65.624 3 mg of total amino acids, 6.301 0 mg of free amino acid, 59.323 3 mg of peptides in one milliliter. Besides, the attractive effect of hydrolyzed protein was stronger than before. The results can provide a basis for monitoring protein bait quality on the industrial production.

关 键 词：橘小实蝇 蛋白饵剂 游离氨基酸 总氨基酸 高效液相色谱 

分 类 号：S482.2[农业科学—农药学]