免疫健全小鼠Panc02胰腺癌发展过程中免疫细胞群的动态变化  

Dynamic Changes of Immune Cell Populations in Panc02 Pancreatic Cancer Bearing Immunocompetent Mice

作　　者：刘乔飞[1] 廖泉[1] 宗毅[1] 牛哲禹[1] 王梦一[1] 李媛[2] 卢朝辉[2] 赵玉沛[1] 

机构地区：[1]中国医学科学院北京协和医院基本外科,北京100730 [2]中国医学科学院北京协和医院病理科,北京100730

出　　处：《中国普外基础与临床杂志》2014年第11期1366-1371,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金面上资助项目(编号:81272573)~~

摘　　要：目的了解免疫健全小鼠胰腺癌发展过程中免疫细胞群的动态变化情况。方法利用C57BL6/J小鼠Panc02同源胰腺癌细胞株建立免疫健全小鼠胰腺癌种植瘤模型。根据肿瘤大小将其分为T1～T4期。流式细胞染色计数胰腺癌发展过程中炎细胞（CD45＋）、辅助T（Th）淋巴细胞、细胞毒性T淋巴细胞（CTL）、B淋巴细胞、粒细胞、巨噬细胞、树突细胞（DC）、自然杀伤（NK）细胞及自然杀伤T（NKT）细胞在外周血及肿瘤组织中的动态变化。结果随着小鼠胰腺癌进展,免疫细胞群比例分别呈现＂持续减少＂、＂持续增多＂及＂先增多后减少＂3种变化形式。1外周血中,Th淋巴细胞、CTL及B淋巴细胞比例逐渐减少;粒细胞比例持续增多;DC、巨噬细胞、NK细胞及NKT细胞比例在T1～T3期逐渐增多但在T4期骤然减少。2肿瘤组织中,总炎细胞浸润逐渐增多;粒细胞、DC及巨噬细胞比例逐渐增多;Th淋巴细胞及CTL比例逐渐减少;B淋巴细胞比例无明显变化;NK细胞及NKT细胞比例在T1～T3期逐渐增多但在T4期骤然减少。结论随着小鼠胰腺癌进展,外周血及肿瘤组织中各免疫细胞群呈现不同的动态变化趋势,提示不同免疫细胞群在预测胰腺癌预后及综合治疗中可能起重要作用。Objective To explore the dynamic changes of immune cell populations in Panc02 pancreatic cancer bearing immunocompetent mice. Methods The C57BL6/J mice syngeneic pancreatic cancer cell line Panc02 were subcutaneously implanted to establish the immunocompetent murine pancreatic cancer bearing model. According to the tumor size, the tumor was classified into 4 stages, named Tt-T4, respectively. The flow cytometry was performed to identify the dynamic changes of different cell populations, such as inflammatory cells （CD45＋） , T helper （Th） lymphocytes, cytotoxic T lymphocytes （CTL）, B lymphocytes, granulocytes, macrophages, dendritic cells （DC）, natural killer （NK） cells, and natural killer T （NKT） cells in the peripheral blood and tumor tissue. Results Three dynamic types of immune cells with the tumor progression were identified： consistent increase, consistent decrease, increase and then decrease. ① In peripheral blood： The proportion of the Th lymphocytes, CTL, and B lymphocytes consistently decreased; The proportion of granulocytes consistently increased; The proportion of the DC, macrophages, NK cells, and NKT cells increased from T1 to T3 stage but sharply decreased at T4 stage. ② In tumor tissue： The intratumoral CD45＋ cells consis- tently increased; The proportion of the granucolyte, macrophages, and DC consistently increased; The proportion of the Th lymphocytes and the CTL consistently decreased; The proportion of the B lymphocytes did not change significantly; The proportion of the NK cells or NKT cells increased from T1 to T3 stage but sharply decreased at T4 stage. Conclusion With pancreatic cancer progression, the immune cell populations show different dynamic change models, which imply their important roles in predicting the prognosis and the integrated treatments of pancreatic cancer.

关 键 词：免疫健全小鼠 胰腺癌 免疫细胞群 综合治疗 预后 

分 类 号：R735.9[医药卫生—肿瘤]