RNA干扰畸胎瘤细胞源性生长因子基因对人肝细胞癌HepG2增殖和侵袭的影响  

INTERFERENCE OF PC CELL-DERIVED GROWTH FACTOR RNA ON PROLIFERATION AND INVASION OF HUMAN HEPATOCELLULAR CARCINOMA CELL LINE HEPG2

作　　者：王海燕[1] 黄爱民[1] 晋雯[1] 高凌云[1] 高美钦[1] 

机构地区：[1]福建医科大学基础医学院病理学系,福建医科大学肿瘤研究室,福建福州350004

出　　处：《河北医科大学学报》2014年第11期1291-1295,F0002,共6页Journal of Hebei Medical University

基　　金：福建省教育厅课题(JA10133);福建省卫生厅青年科研课题(2010-1-19)

摘　　要：目的探讨RNA干扰畸胎瘤细胞源性生长因子(PC cell-derived growth factor,PCDGF)基因对肝细胞癌细胞HepG2增殖和侵袭的影响。方法采用脂质体法将PCDGF-shRNA表达载体瞬时转染人肝细胞癌细胞HepG2,逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和免疫蛋白印迹(Western Blot)检测转染后细胞PCDGF mRNA和蛋白的表达,噻唑蓝(thiazolyl blue tetrazolium bromide,MTT)比色法检测转染后HepG2细胞体外增殖能力,体外侵袭实验检测转染后HepG2细胞侵袭能力。结果转染后,与未转染组(未转染质粒和脂质体)、脂质体组(转染空脂质体)和阴性质粒组(转染阴性质粒)相比,转染组(转染PCDGF-shRNA质粒)HepG2细胞的PCDGF mRNA和蛋白表达水平明显降低(P<0.01),细胞的增殖能力受到抑制(P<0.01),细胞的侵袭能力也明显降低(P<0.01)。结论 RNA干扰PCDGF基因可抑制肝细胞癌细胞的体外增殖及侵袭能力,PCDGF基因可能成为肿瘤基因治疗的新靶点。Objective To investigate the effect of PC cell-derived growth factor（PCDGF） RNA on hepatocellular carcinoma cells HepG2 in vitro .Methods The PCDGF-shRNA expression vector was transfected into hepatocellular carcinoma cells HepG2 by lipofection technology.The expression of PCDGF mRNA and protein were detected by reverse transcription-polymerase chain reaction（RT-PCR）and Western Blot,respectively.The ability of tumor cell proliferation was determined by MTT assay.The effect of PCDGF expression on invasion was also identified by transwell chamber system.Results The expression of PCDGF mRNA and protein were both decreased in the transfection group than in the non-transfection group,liposome group and negative vector group（P 〈0.01）.The MTT showed cell proliferation activity was inhibited in the transfection group （P 〈0.01）.Transwell experiments showed that cell invasion ability was lower in the transfection group compared with that in the non-transfection,liposome and negative vector＆amp;nbsp;groups（P 〈0.01）.Conclusion PCDGF RNA interference can inhibit the proliferation and invasion abilities of hepatocellular carcinoma cells in vitro .PCDGF gene may be the new target of gene therapy.

关 键 词：肝肿瘤 RNA干扰 细胞增殖 肿瘤浸润 

分 类 号：R735.7[医药卫生—肿瘤]