恩施产杜仲药材HPLC指纹图谱的研究  

Study on Fingerprint of Eucommia Ulmoides from Enshi by HPLC

作　　者：周妍[1] 刘大鹏[1,2] 王薇[1,2] 詹琤琤[1] 李杰[1] 周才新[1,2] 范彦博[1,2] 

机构地区：[1]武汉市中医医院,武汉430014 [2]武汉市中医药研究所国家中医药管理局中药制剂三级实验室

出　　处：《中国药师》2014年第12期2071-2074,共4页China Pharmacist

基　　金：武汉市卫生与计划生育委员会课题(编号:WZ13Z01)

摘　　要：目的：建立恩施产杜仲药材水提液HPLC指纹图谱的分析方法。方法：10批恩施产杜仲药材水提,采用Wonda Sil C18（250 mm×4.6 mm,5μm）色谱柱,以乙腈-0.1%磷酸水溶液二元线性梯度洗脱,检测波长230 nm,流速1.0 ml·min^-1,柱温25℃,进样量10μl,进行HPLC指纹图谱分析。结果：确定了12个共有峰,采用《中药色谱指纹图谱相似度评价系统（2004A版）》计算10批杜仲药材水提液的指纹图谱整体相似度在0.596-0.997,并得出对照指纹图谱。结论：针对恩施产杜仲药材建立的HPLC指纹图谱分析方法,操作简便,稳定性高,重复性好,可有效地控制恩施产杜仲药材的内在质量。Objective： To establish the fingerprint analysis method for the aqueous extracts of Eucommia ulmoides from Enshi by HPLC. Methods： The fingerprint of aqueous extracts of ten batches of Eucommia ulmoides from Enshi were analyzed by HPLC. The column was Wonda Sil C18（ 250 mm × 4. 6 mm,5 μm）. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min^-1,the detection wavelength was 230 nm,the column temperature was 25℃,and the injection volume was 10 μl. Results： The fingerprint consisted of 12 common peaks. The similarity range of ten batches of Eucommia ulmoides calculated by similarity evaluation system for the chromatographic fingerprint of TCM（ Version 2004 A） was 0. 596-0. 997. The standard fingerprint of Eucommia ulmoides was established by HPLC. Conclusion： The established HPLC fingerprint analysis method for Eucommia ulmoides from Enshi is simple,stable and reproducible,which can effectively control the quality of Eucommia ulmoides from Enshi.

关 键 词：杜仲 高效液相色谱 指纹图谱 共有模式 恩施 

分 类 号：TQ460.72[医药卫生—药物分析学]