替莫唑胺对胶质瘤侵袭性的影响及可能机制  

Effect of temozolomide on the glioma invasiveness and its possible mechanism

作　　者：王健[1] 贾永森[2] 赵喜庆[3] 秦丽娟[1] 

机构地区：[1]河北联合大学基础医学院生理学教研室,唐山063000 [2]河北联合大学中医学院,唐山063000 [3]河北联合大学附属唐山市人民医院神经外科,唐山063000

出　　处：《临床与实验病理学杂志》2014年第11期1259-1262,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省卫生厅科研基金(20100465;20110165;20120144);河北联合大学培育基金(SP201310)

摘　　要：目的探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在替莫唑胺(temozolomide,TMZ)降低胶质瘤侵袭性过程中的作用及机制。方法对数生长期的C6胶质瘤细胞随机分为TMZ处理10、30、60、120、180和240 min组,每组各15例,动态监测培养液中TNF-α的含量(放射免疫法)、C6细胞内p53蛋白的表达(Western blot法)和细胞凋亡水平(Annexin V-FITC法)。制备体外胶质瘤的侵袭模型,利用结晶紫染色法检测胶质瘤的侵袭性。结果 TMZ作用于C6细胞后,培养液中TNF-α的含量明显增加,于120 min时含量最多(P<0.01),其后开始减少。C6细胞内p53蛋白的表达于处理后120 min达高峰(P<0.01),其后逐渐减少。TMZ作用于体外胶质瘤侵袭模型后,胶质瘤细胞的侵袭性降低。结论 TNF-α介导TMZ降低胶质瘤侵袭性,此作用可能是由于TMZ促进C6细胞释放TNF-α,增加的TNF-α又促进胶质瘤细胞凋亡所致。Purpose To explore the role of tumor necrosis factor-α（ TNF-α） in the process of temozolomide（ TMZ） reduce glioma invasiveness and its possible mechanism. Methods C6 glioma cells of logarithmic phase were randomly divided into TMZ treatment（ 10,30,60,120,180,240 min group）（ n = 15）,dynamic monitoring content of TNF-α in the culture medium was measured by radioimmunoassay,expression of p53 protein in C6 cells was detected with Western blotting method,and cell apoptosis was used with Annexin V-FITC. A glioma invasiveness model was established in vitro and glioma invasiveness was determined by crystal violet staining. Results For C6 cells,contents of TNF-α in the nutrient fluid and expressions of p53 protein in C6 cells obviously increased after TMZ treatment and they achieved the peak at 120 min（ P 0. 01）,followed by decrease gradually. Glioma invasiveness was reduced after TMZ acted on glioma in vitro. Conclusion TMZ can reduce glioma invasiveness by TNF-α,which this role may be is TMZ promote C6 cells release of TNF-α and increased TNF-α due to glioma cells apoptosis.

关 键 词：胶质瘤 肿瘤坏死因子-Α 替莫唑胺 侵袭性 凋亡 P53 

分 类 号：R739.4[医药卫生—肿瘤]