胃黏膜细胞中Foxp3、TGF-β1、IL-10的表达及其与幽门螺杆菌感染的关系  

Expression of Foxp3,TGF-β1 and IL-10 in the gastric mucosa of patients with Helicobacter pylori infection

作　　者：苏国娟 郭彦言[1] 

机构地区：[1]唐山市丰南区医院检验科,河北省唐山市063300

出　　处：《世界华人消化杂志》2014年第32期4964-4968,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨幽门螺杆菌(Helicobacter pylori,H.pylori)感染与胃黏膜细胞叉头蛋白3(fork head3,F oxp3)、肿瘤生长因子-β1(tumor grow th factor-β1,TGF-β1)、白介素-10(interleukin-10,IL-10)表达的关系.方法:按照病理检查结果将100例胃镜检查患者分为实验组(胃癌)30例和对照组(慢性浅表性胃窦炎、慢性萎缩性胃炎、胃溃疡、十二指肠溃疡)70例,比较两组患者H.pylori感染情况及胃黏膜细胞Foxp3、TGF-β1及IL-10的表达.结果:本组100例患者H.p y l o r i阳性72例(72.00%),实验组感染率为63.33%,对照组感染率为75.71%,总感染率为72.00%;Foxp3m R N A在实验组和对照组阳性表达率比较(73.33%vs 54.29%),差异无统计学意义(P>0.05);Foxp3 m RNA在H.pylori感染阳性组阳性表达率与H.pylori感染阴性组阳性表达率比较(68.06%vs 39.29%),差异具有统计学意义(P<0.05);TGF-β1 m RNA在实验组和对照组阳性表达率比较(73.33%vs 62.86%),差异无统计学意义(P>0.05);TGF-β1 m RNA在H.pylori感染阳性组阳性表达率与H.pylori感染阴性组阳性表达率比较,(75.00%vs 42.86%),差异具有统计学意义(P<0.05);两组患者H.pylori阳性IL-10蛋白含量均显著高于H.pylori阴性(3.58 pg/m L±0.65 pg/m L vs 0.58 pg/m L±0.03 pg/m L,2.84 pg/m L±0.89 pg/m L vs 0.97p g/m L±0.22 p g/m L),差异具有统计学意义(P<0.01).结论:H.pylori感染后,可诱导Treg细胞Foxp3的高表达以及TGF-β1、IL-10表达上调,抑制机体胃黏膜局部免疫功能,为H.pylori的持续性感染提供了微环境.AIM： To explore the expression of fork head 3 （Foxp3）, tumor growth factor-β1 （TGF-β1） and interleukin-10 （IL-10） in the gastric mucosa of pa- tients with Helicobacter pylori （H. pylori） infection. METHODS： One hundred patients who under- went gastroscopy were divided into either an experiment group （patients with gastric cancer, n = 30） or a control group （patients with chronic superficial antral inflammation, chronic atrophic gastritis, gastric ulcer, or duodenal ulcer, n = 70） according to the operative and pathological find- ings. H. pylori infection status and the expression of Foxp3, TGF-β1 and IL-10 were compared for the two groups. RESULTS： Of the 100 patients, 72 （72.00%） were positive forH, pylori infection. The rates of H. py-lori infection for the experiment group and con- trol group were 63.33% and 75.71%, respectively. Although there were no significant differences in the positive expression rates of Foxp3 and TGF-β1 mR_NAs between the experiment group and control group （73.33% vs 54.29%, 73.33% vs 62.86%, P 〉 0.05）, significant differences were noted between the H. pylori infection positive group and negative group （68.06% vs 39.29%, 75.00% vs 42.86%, P 〈 0.05）. The protein level of IL-10 differed significantly between the experi- ment group and control group and between the H. pylori infection positive negative groups （3.58 pg/mL ± 0.65 pg/mL vs 0.58 pg/mL ± 0.03 pg/ mL, 2.84 pg/mL ± 0.89 pg/mL vs 0.97 pg/mL ± 0.22 pg/mL, P 〈 0.01）. CONCLUSION： H. pylori infection may directly or indirectly induce the expression of TGF-131, IL-10 and Foxp3, which can inhibit the immunity in the gastric mucosa and thus provide a micro- environment for sustained H. pylori infection.

关 键 词：幽门螺杆菌 胃黏膜 叉头蛋白3 肿瘤生长 因子-β1 白介素-10 

分 类 号：R573[医药卫生—消化系统]