JAK2-STAT3通路介导肢体缺血后处理脑缺血再灌注损伤保护作用的研究  

Research the protective effect of JAK2-STAT3 pathway mediated limb ischemia treatment of cerebral ischemia-reperfusion injury

作　　者：韦家俊[1] 李浩[1] 廖小明[1] 齐立[1] 吴岚[1] 刘开祥[1] 

机构地区：[1]桂林医学院附属医院神经内科,桂林541001

出　　处：《安徽医科大学学报》2014年第12期1714-1717,共4页Acta Universitatis Medicinalis Anhui

基　　金：2011桂林市科技开发项目(编号:0110119-1-6);2012广西省自然科技基金(编号:2012jj AA40148)

摘　　要：目的探讨肢体缺血后处理对大鼠脑缺血再灌注损伤后脑保护的作用机制。方法健康雄性SD大鼠48只,随机分为4组即假手术组(sham)、缺血再灌注组(I/R组)、肢体缺血后处理组(Lpost组)、肢体缺血后处理+AG490组(AG组)。I/R组、Lpost组、AG组均做缺血2 h再灌注24 h,Lpost组:再灌注前实施肢体缺血后处理(缺血15 min,灌注15 min)3个循环,AG组:再灌注前5 min时腹腔注射AG490(l mg/kg),其他处理与Lpost组相同。各组大鼠神经功能评分后,采用TTC染色测脑梗死体积,TUNEL法测定脑细胞凋亡。结果 1与sham组相比,I/R组大鼠神经功能缺损加重(P<0.05);与I/R组相比,Lpost组大鼠神经功能缺损减轻(P<0.05);与Lpost组相比,AG组大鼠神经功能缺损加重(P<0.05);2 sham组大鼠无梗死灶,与I/R组相比,Lpost组大鼠梗死体积减小(P<0.05);与Lpost组相比,AG组梗死体积增大(P<0.05);3与I/R组相比,Lpost组大鼠脑细胞凋亡率明显降低(P<0.05);与Lpost组相比,AG组脑细胞凋亡率明显增高(P<0.05)。结论肢体缺血后处理具有显著的脑保护作用,这一作用是由JAK2-STAT3通路介导的。Objective To investigate the mechanism of limb ischemia after treatment on cerebral ischemia-reperfusion injury of brain protection.Methods Forty-eight male Sprague-Dawley rats were randomly divided into four groups namely sham group （sham）,ischemia-reperfusion （I/R group）,Limb ischemia treatment （Lpost group）,Limb ischemia treatment ＋ AG490 group （AG group）.I/R group,Lpost group,AG group were doing ischemia 2 h,reperfusion 24 h,Lpost group：then after the implementation of limb ischemia before reperfusion treatment （ischemia 15 min,perfusion 15 min） 3 cycles,AG group：before 5 min reperfusion intraperitoneal injection of AG490 （1 mg/kg）,the same as the other dealing with Lpost groups.The rats neurological score,using TTC staining to measure infarct volume,using TUNEL to detect neural cell apoptosis.Results ① Compared with the sham group,I/R rats neurological deficit increased （P ＜ 0.05） ; compared with the I/R group,Lpost alleviated neurological deficit rats （P ＜ 0.05） ; compared with the Lpost group,AG group of neurological defects in rats increased （P ＜ 0.05） ; ② sham rats without infarct volume,compared with the I/R group,Lpost rats reduced infarct volume （P ＜ 0.05） ; compared with Lpost group,AG infarct volume increased （P ＜ 0.05） ; ③ with I/R group rats brain cells apoptosis,Lpost index was significantly lower （P ＜ 0.05） ; compared with Lpost group,AG group of brain cells wither death Index was significantly higher （P ＜ 0.05）.Conclusion limb ischemia treatment has a significant neuroprotective effect,this effect is mediated by the JAK2-STAT3 pathway.

关 键 词：脑缺血再灌注损伤 肢体缺血后处理 JAK2-STAT3 细胞凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]