HSP90α、HSP90β在人胃癌组织中的表达  

Expression and clinical significance of HSP90α,HSP90β protein and mRNA in human gastric carcinoma

作　　者：吴梦婕[1] 张红[1] 姚元春[2] 秦蓉[1] 

机构地区：[1]安徽医科大学基础医学院病理学教研室,合肥230032 [2]吉首大学医学院病理学教研室,吉首416000

出　　处：《安徽医科大学学报》2014年第12期1754-1758,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:090413118)

摘　　要：目的探讨HSP90α、HSP90β蛋白与mRNA在人胃癌组织中的表达及其与胃癌生物学行为的关系。方法采用免疫组化SP法检测两者蛋白在胃癌组织及癌旁正常组织的表达。采用RT-PCR法检测两者的mRNA在胃癌组织及癌旁正常组织的表达。结果 HSP90α、HSP90β主要定位于细胞质,其阳性率分别为82.9%和80.0%(P<0.01),与胃癌分化、浸润、分期、转移相关。HSP90α、HSP90βmRNA在胃癌中的表达明显高于癌旁正常组织(P<0.01),并与胃癌的分化、分期、淋巴结的转移相关。结论 HSP90α、HSP90β蛋白在胃癌组织中的阳性表达以及其与胃癌生物学行为的关系提示HSP90α、HSP90β可能作为判断胃癌恶性程度的重要指标。HSP90α、HSP90βmRNA的高表达,且两者均与胃癌的发生、发展关系密切,可能与胃癌组织中的转录调控相关。Objective To investigate the expression of protein and mRNA of HSP90α,HSP90β in human gastric carcinoma and normal mucosa,and to determine their relation to biological behaviors of gastric carcinoma.Methods The protein expression of HSP90α and HSP90β in gastric carcinoma tissues and the adjacent normal tissues were detected by immunohistochemical SP method.The expressions of HSP90α mRNA and HSP90β mRNA in gastric carcinoma tissues and the adjacent normal tissues were detected by reversing transcription-polymerase chain reaction （RT-PCR） method.Results The positive expressions of HSP90α and HSP90β protein were mainly located in cytoplasm,the positive expression rates were 82.9％ and 80.0％ （P ＜0.01） respectively.It was related to differentiation,depth of tumor invasion,stage,metastasis of gastric cancer.The expressions of HSP90α and HSP90β mRNA in gastric carcinoma were significantly higher than the adjacent normal tissues （P ＜ 0.01）,which was related to the differentiated degree,the stage and lymphatic metastasis.Conclusion The positive expressions of HSP90α and HSP90β protein in gastric carcinoma,as well as their relationship with biological behaviors of gastric carcinoma indicate that HSP90α and HSP90β act as critical indicators that contribute to the malignancy of gastric carcinoma.Both the high expressions of HSP90α mRNA and HSP90β mRNA,which are closely related to the occurring and development of gastric carcinoma,may have relationship with the transcriptional regulation in the gastric carcinoma tissues.

关 键 词：HSP90Α HSP90β 免疫组织化学 逆转录聚合酶链式反应 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤] R341.32[医药卫生—临床医学]