乙型肝炎病毒相关性肝细胞癌患者血清微RNA-574-3p的表达及其意义  

Expression and its significance of serum microRNA-574-3p in hepatitis B virus-related hepatocellular carcinoma

作　　者：薛亚晶[1] 陈建[2] 施维[3] 张金业[1] 张慧[1] 鞠少卿[3] 

机构地区：[1]南通市肿瘤医院检验科,江苏省226000 [2]南通市第三人民医院消化内科 [3]南通大学附属医院外科综合实验室

出　　处：《中华消化杂志》2014年第11期732-736,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金面上项目（81271920）;江苏省“科技兴卫工程”创兴团队项目（LJ201133）;南通市科技局指导课题（HS13922）

摘　　要：目的 探讨HBV相关性肝细胞癌患者血清微RNA-574-3p的表达水平及其辅助诊断的价值.方法 纳入90例HBV相关性肝细胞癌患者、40例HBV相关性肝硬化患者和50名健康对照者.检测所有受试者血清标本中微RNA-574-3p的表达水平、AFP和α-L-岩藻糖苷酶（AFU）的含量.比较不同组间血清微RNA-574-3p水平的差异,分析肝细胞癌患者血清微RNA-574-3p水平与其临床病理特征之间的关系,均采用t检验.采用Spearman相关性分析肝细胞癌患者血清微RNA-574-3p水平与AFP,AFU的关系,应用ROC曲线及AUC（95％CI）评估它们作为诊断指标的诊断效能.结果 肝细胞癌组、肝硬化组与健康对照组血清微RNA-574-3p的相对表达量分别为2.152（1.654,3.061）、1.292（0.984,1.666）和1.018（0.750,1.726）.肝细胞癌患者血清微RNA-574-3p表达量高于肝硬化患者和健康对照者,差异均有统计学意义（t=2.726,2.845;P均＜0.01）.肝细胞癌患者血清微RNA-574-3p相对表达量在不同的分化程度（t=2.262,P=0.039）、肿瘤分期（t=2.354,P=0.025）和HBVDNA含量（t=2.771,P＜0.01）间差异均有统计学意义.肝细胞癌患者血清微RNA-574-3p相对表达量与AFP,AFU之间无相关性（r2=0.076,P=0.505;r2=0.082,P=0.422）.肝细胞癌组与肝硬化组比较,血清微RNA-574-3p的AUC为0.823,95％CI为0.750～0.897;肝细胞癌组与健康对照组比较,血清微RNA-574-3p的AUC为0.840,95％CI为0.769～0.910.结论 肝细胞癌患者血清微RNA-574-3p含量高于肝硬化患者和健康对照者.血清微RNA-574-3p可能是一个重要的诊断肝细胞癌的生物学指标.Objective To explore the expression and diagnostic value of serum microRNA-574-3p （miR-574-3p） in hepatitis B virus （HBV）-related hepatocellular carcinoma （HCC）.Methods A total of 90 patients with HBV-related HCC,40 patients with HBV-related liver cirrhosis （LC） and 50 healthy controls were recruited.The expression levels of serum miR-574-3p,α-fetoprotein （AFP） and α-Lfucosidase （AFU） of all subjects were determined.The difference of serum miR-574-3p level between groups was compared.The relation between serum miR-574-3p level of HCC patients and its clinical pathological characteristics was analyzed.The t-test was performed.The relationship between serum miR-574-3p level of HCC patients and AFP,AFU was analyzed by Spearman correlation analysis.The diagnostic efficacy of them as diagnostic markers was evaluated by receiver operating characteristic curves （ROC） and the area under the curve （AUC）（95％ CI （confidence interval））.Results The relative quantity expression of serum miR-574-3p of HCC group,LC group and healthy group was 2.152（1.654,3.061）,1.292 （0.984,1.666） and 1.018 （0.750,1.726）,respectively.The expression level of serum miR-574-3p of HCC group was significantly higher than those of LC group and healthy control group,and the differences were statistically significant （t=2.726 and 2.845,both P＜0.01）.The expression level of serum miR-574-3p of HCC patients was significantly different in tumors with different degree of differentiation （t=2.262,P=0.039）,different stage （t=2.354,P=0.025） and different HBV DNA concentrations （t=2.771,P＜0.01）.There was no correlation between serum miR-574-3p level and AFP （r2 =0.076,P=0.505）,AFU （r2 =0.082,P=0.422） in HCC patients.When compared HCC group with LC group,AUC of serum miR-574-3p of was 0.823 and 95％ CI was 0.750 to 0.897.When compared HCC group with healthy control group,AUC of serum miR-574-3p was 0.840 and 95％CI was 0.769 to 0.910.Conclusions The expression level of serum miR

关 键 词：肝炎病毒 乙型 癌 肝细胞 微RNA-574-3p 

分 类 号：R735.7[医药卫生—肿瘤]