绿色木霉菌H06固体浅盘发酵工艺优化  

Optimization of shallow tray fermentation process of Trichoderma vi-ride H06

作　　者：梁昌聪[1] 刘磊[1] 张建华[1] 郭立佳[1] 杨腊英[1] 王国芬[1] 王伟伟[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物检测与控制重点实验室,海南海口571101

出　　处：《菌物学报》2014年第6期1313-1326,共14页Mycosystema

基　　金：国家公益性行业(农业)科研专项(No.200903049)

摘　　要：对前期筛选获得防治香蕉枯萎病效果较好的绿色木霉菌菌株Trichoderma viride H06,通过Plackett‐Burman试验设计及Box‐Behnken设计的响应曲面法(response surface methodology,RSM)对影响生防菌株H06固体发酵培养基和培养条件进行了优化。确定固体培养基3个主要因子的最佳用量分别为锯末1.05kg、玉米粉0.72kg、KH2PO4 28.24g。培养条件优化试验得出:培养含水量、培养基厚度和温度是影响固体发酵产物孢子含量的主要因子,由所得响应曲面方程预测出这3个主要因子分别为51.26%、3.27cm和28.38℃时,在培养时间6d、接种量10%、菌龄60h、初始p H为6的条件下,固体发酵产物最大预测值为3.24×1010个/g。经验证,该理论预测值与实际值相近。The Plackett-Burman experiments and response surface methodology were adopted to optimize the so mentation medium and conditions of the biocontrol agent of Trichoderma viride H06, According to the evaluation response surface model established in this study, the optimum mass concentrations of the three components, sa corn meal and KH2PO4, in fermentation medium were 1.05kg, 0.72kg and 28.24g, respectively. The fermentation lid fer- by the wdust, condi-tion determination tests indicated that water-material ratio, thickness of medium and temperature were the major fac- tors affecting H06 yield. The optimized fermentation conditions were water-material ratio 51.26%, thickness of medium 3.27cm and temperature 28.38℃ for 6d, inoculum volume 10%, spawn age 60h and initial pH7.0. Under the conditions, the maximum theoretic spore production was 3.24×lO^10/g. Fermentation experiments verified that there was consistent between forcasted and real yields.

关 键 词：绿色木霉菌 Plackett.Burman设计 响应面法 最适培养条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] S476.1[农业科学—农业昆虫与害虫防治]