实验性视网膜脱离复位后脑源性神经营养因子对视网膜的保护作用  

The neuroprotection of brain-derived neurotrophic factor in experimental retinal detachment reattached

作　　者：曾爱萍[1] 徐海艳[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022 [2]云南省曲靖市第一人民医院眼科

出　　处：《中华眼科杂志》2014年第11期820-825,共6页Chinese Journal of Ophthalmology

基　　金：湖北省自然科学基金（2010CDB7802）

摘　　要：目的 探讨实验性视网膜脱离（RD）复位后外源性脑源性神经营养因子（BDNF）对RD损伤后视网膜修复的影响.方法 实验研究.选用健康SD大鼠48只,随机分为BDNF实验组、实验对照组、RD复位组、正常对照组.采用光镜和电镜观察RD复位后视网膜形态和视网膜细胞超微结构,检测BDNF对RD复位后视网膜变性的影响;采用原位缺口末端转移酶标记法（TUNEL）检测BDNF对RD复位后视网膜细胞凋亡的影响.组间均数比较采用方差分析,进一步两两比较采用t检验.结果 视网膜自动复位平均时间为（31.3±3.5）d.光镜、电镜观察BDNF实验组与实验对照组相比,视网膜内外节损伤轻,内、外核层细胞数多,外核层厚,视网膜结构和层次排列好.BDNF实验组和实验对照组内核层厚度分别为（21.166±3.087）和（16.084±2.928） μm,外核层厚度分别为（23.508±3.679）和（12.885±3.070） μm,两组差异均有统计学意义（t=3.776,6.996;P＜0.01）.BDNF实验组视网膜外核层凋亡细胞数少于实验对照组.结论 RD复位后视网膜形态及视网膜细胞超微结构发生了改变,视网膜细胞凋亡.外源性BDNF能减轻RD复位后视网膜细胞的变性及凋亡,对PD复位后视网膜损伤有一定的保护作用.Objective To investigate the effect of exogenous brain-derived neurotrophic factor （BDNF） on the retinal repair after experimental retinal detachment （RD） reattached.Methods Experimental study.Forty-eight normal rats were randomly divided into four groups：BDNF group,control group,RD reattached group and normal group.In order to detect the effects of BDNF on retinal degeneration caused by RD,the morphology of retina and the ultrastructure of retinal cells were observed by light microscopy and electron microscopy.The effect of BDNF on the apoptosis of retinal cells after RD reattached was detected by Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling （TUNEL） technique.Data were analyzed using one-way analysis of variance （ANOVA） and secondary analysis for significance with independent-samples t-test.Results The average automatically reattached time was （31.3 ± 3.5）days.Compared with control group,there were less damage in out segment and inner segment,more retinal cells in outer nuclear layer （ONL） and inner nuclear layer （INL）,thicker ONL of retina and better organizational structure of retina in BDNF group.The average thickness of ONL and INL of retina were （21.166 ± 3.087） μm,（23.508 ± 3.679） μm respectively in BDNF group,and （16.084 ± 2.928）μm,（12.885±3.070） μm respectively in control group.The thickness of ONL and INL of retina showed a significant difference in BDNF group compared with control （P 〈 0.01）.There were less TUNEL-positive cells in ONL of retina in BDNF group compared with control.Conclusions This study indicates that the morphology of retina and the ultrastructure of retinal cells have changed,and the apoptosis of retinal cells are present after RD reattached.Exogenous BDNF can reduce retinal cells degeneration and inhibit the apoptosis of retinal cells and have a certain protection effects on the retinal damage caused by RD.

关 键 词：视网膜脱离 脑源性神经营养因子 视网膜神经节细胞 细胞凋亡 大鼠 

分 类 号：R774.1[医药卫生—眼科]