Working conditions of bipolar radiofrequency on human articular cartilage repair following thermal injury during arthroscopy  

作　　者：Huang Yuelong Zhang Yujun Ding Xiaoquan Liu Songyang Sun Tiezheng 

机构地区：[1]Arthritis Clinic and Research Center,Peking University People's Hospital, Beijing 100044, China [2]Clinical Institute of Molecular Biology,Peking University People's Hospital, Beijing 100044, China

出　　处：《Chinese Medical Journal》2014年第22期3881-3886,共6页中华医学杂志（英文版）

摘　　要：Background The thermal injury during bipolar radiofrequercy results in chondrocyte death that limits cartilage repair.The purpose was to determine the effects of various factors of bipolar radiofrequency on human articular cartilage after thermal injury,offering suitable working conditions for bipolar radiofrequency during arthroscopy.Methods Osteochondral explants from 28 patients undergoing total knee arthroplasty （TKA） in Department of Orthopaedic,Peking University Reople＇s Hospital from October 2013 to May 2014,were harvested and treated using bipolar radiofrequency in a light contact mode under the following conditions：various power setting of levels 2,4 and 6; different durations of 2 seconds,5 seconds and 10 seconds; irrigation with fluids of different temperatures of 4℃,22℃,and 37℃; two different bipolar radiofrequency probes ArthroCare TriStar 50 and Paragon T2.The percentage of cell death and depth of cell death were quantified with laser confocal microscopy.The content of proteoglycan elution at different temperatures was determined by spectrophotometer at 530 nm.Results Chondrocyte mortality during the treatment time of 2 seconds and power setting of level 2 was significantly lower than that with long duration or in higher level groups （time：P=0.001; power：P=0.001）.The percentage of cell death after thermal injury was gradually reduced by increasing the temperature of the irrigation solutions （P=0.003）,the depth of dead chondrocytes in the 37℃ solution group was significantly less than those in the 4℃ and 22℃ groups （P=0.001）.The proteoglycan elution was also gradually reduced by increasing the temperature （P=0.004）.Compared with the ArthroCare TriStar 50 group,the percentage of cell death in the Paragon T2 group was significantly decreased （P=0.046）.Conclusions Thermal chondroplasty with bipolar radiofrequency resulted in defined margins of chondrocyte death under controlled conditions.The least cartilage damage during thermal chondroplasty could be achieveBackground The thermal injury during bipolar radiofrequercy results in chondrocyte death that limits cartilage repair.The purpose was to determine the effects of various factors of bipolar radiofrequency on human articular cartilage after thermal injury,offering suitable working conditions for bipolar radiofrequency during arthroscopy.Methods Osteochondral explants from 28 patients undergoing total knee arthroplasty （TKA） in Department of Orthopaedic,Peking University Reople＇s Hospital from October 2013 to May 2014,were harvested and treated using bipolar radiofrequency in a light contact mode under the following conditions：various power setting of levels 2,4 and 6; different durations of 2 seconds,5 seconds and 10 seconds; irrigation with fluids of different temperatures of 4℃,22℃,and 37℃; two different bipolar radiofrequency probes ArthroCare TriStar 50 and Paragon T2.The percentage of cell death and depth of cell death were quantified with laser confocal microscopy.The content of proteoglycan elution at different temperatures was determined by spectrophotometer at 530 nm.Results Chondrocyte mortality during the treatment time of 2 seconds and power setting of level 2 was significantly lower than that with long duration or in higher level groups （time：P=0.001; power：P=0.001）.The percentage of cell death after thermal injury was gradually reduced by increasing the temperature of the irrigation solutions （P=0.003）,the depth of dead chondrocytes in the 37℃ solution group was significantly less than those in the 4℃ and 22℃ groups （P=0.001）.The proteoglycan elution was also gradually reduced by increasing the temperature （P=0.004）.Compared with the ArthroCare TriStar 50 group,the percentage of cell death in the Paragon T2 group was significantly decreased （P=0.046）.Conclusions Thermal chondroplasty with bipolar radiofrequency resulted in defined margins of chondrocyte death under controlled conditions.The least cartilage damage during thermal chondroplasty could be achieve

关 键 词：bipolar radiofrequency articular cartilage CHONDROCYTES thermal injury 

分 类 号：R687.4[医药卫生—骨科学]