消癌平与奥沙利铂及恩度联合对人肝癌细胞株SMMC-7721的体外增殖抑制实验  

Effect of Xiaoaiping, oxaliplatin and Endostar, alone or in combination, on cell proliferation in human hepatoma cell line SMMC-7721

作　　者：马继恒[1] 华海清[2] 戴婷婷[3] 秦叔逵[2] 杨爱珍[2] 

机构地区：[1]丹阳市中医院肿瘤科,江苏省丹阳市212300 [2]中国人民解放军第八一医院全军肿瘤中心,江苏省南京市210000 [3]中国人民解放军南京军区总医院肿瘤科,江苏省南京市210000

出　　处：《世界华人消化杂志》2014年第29期4461-4466,共6页World Chinese Journal of Digestology

基　　金：全军"十一.五"中医药临床重大攻关基金资助项目;No.2006051005~~

摘　　要：目的:通过体外实验研究消癌平(Xiaoaiping,XAP)注射液、奥沙利铂(oxaliplatin,L-OHP)、恩度(Endostar,重组人血管内皮抑制素)单药及其与XAP的两药及三药联合应用对人肝癌SMMC-7721增殖的影响,探讨其联合用药的理论依据.方法:采用MTT法观察XAP、L-OHP、恩度单药及其XAP的两药及三药联合应用对人肝癌SMMC-7721细胞增殖抑制作用,并观察细胞形态的变化.结果:10-40μL/mL XAP和1-4μg/mL L-OHP对SMMC-7721均有明显的抑制作用,并呈时间和浓度依赖性(P<0.01和P<0.05).1-12μg/mL恩度对该细胞无增殖抑制作用.20μL/mL XAP和1μg/mL L-OHP联合对SMMC-7721细胞增殖抑制率分别为82.08%,优于对照组(P<0.01),联合具有相加作用.20μL/mL XAP与6μg/mL恩度联合则具有明显的协同作用.三药联合抑制作用更明显.结论:XAP、L-OHP单药能抑制肝癌细胞增殖;恩度无抑制作用;与XAP两药联合对肝癌细胞增殖有协同增效作用,三药联合作用更明显.AIM： To assess the effect of Xiaoaiping（XAP）,oxaliplatin（L-OHP） and endostatin（Endostar）,alone or in combination, on cell proliferation in human hepatocellular carcinoma（HCC） cell line SMMC-7721.METHODS： MTT assay was used to assess the effect of XAP, L-OHP and Endostar monotherapy, bitherapy or tritherapy on the proliferation of SMMC-7721 cells. Changes in cell morphology were also observed.RESULTS： XAP at concentrations of 10-40 μL/mL and L-OHP at 1-4 μg/mL significantly inhibited SMMC-7721 cell proliferation in a time- and concentration-dependent manner（P〈0.01, P〈0.05）. Endostar at 1-12 μg/mL had no inhibitory effect on cell proliferation. XAP at 20 μL/mL plus L-OHP at 1 μg/mL inhibited SMMC-7721 cell proliferation by 82.08%, significantly better than that in the control group（P〈0.01）, suggesting an additive effect. Endostar at 6 μg/mL combined with 20 μL/mL XAP also had an obvious synergistic effect. Tritherapy resulted in more pronounced inhibition of cell proliferation.CONCLUSION： XAP or L-OHP monotherapy can inhibit the proliferation of SMMC-7721 cells, while Endostar shows no inhibitory effect. XAP combined with either L-OHP or Endostar has a synergistic effect, and tritherapy has more obvious synergistic effect.

关 键 词：消癌平注射液 奥沙利铂 肝癌SMMC-7721细胞株 增殖抑制 

分 类 号：R735.7[医药卫生—肿瘤]