脂质体法与电穿孔法转染两种细胞效率的比较  

Comparison of transfection efficiency of two kinds of cells by lipofection and electroporation

作　　者：张禾璇 单可人 何燕 张婷 王婵娟 官志忠 

机构地区：[1]贵州省妇幼保健院优生遗传科,贵阳550001 [2]贵州省医学分子生物学重点实验室,贵阳550004

出　　处：《重庆医学》2014年第33期4432-4433,共2页Chongqing medicine

基　　金：贵州省科技厅科技计划课题资助(黔科合LG[2011]024);"十二五"国家科技支撑计划项目(2013BAI05B03)

摘　　要：目的对比脂质体与电穿孔法对HepG2、SGC7901/ADM两种细胞的转染效率。方法以HepG2、SGC7901/ADM细胞为研究对象,采用脂质体法和电穿孔法分别转染pcDNA3.1-EGFP质粒,流式细胞仪计算细胞存活率,借助绿色荧光标志蛋白eGFP测算转染效率。结果利用脂质体转染eGFP质粒至HepG2细胞所获得的阳性转染率为(20.8±2.1)%,而电穿孔法转染效率增高至(49.6±2.5)%,二者比较差异有统计学意义(P<0.05)。利用脂质体转染eGFP质粒至SGC7901/ADM细胞所获得的阳性转染率为(25.4±1.3)%,而电穿孔法转染效率增高至(52.6±2.1)%,二者比较差异亦有统计学意义(P<0.05)。结论使用电穿孔法能明显提高大片段载体的转染效率。Objective To compare the transfection efficiency between different transfection methods in human HepG2 and SGC7901/ADM cells so as to provide experimental basis for further study .Methods To electrons fect the enhanced GFP plasmid into HepG2 and SGC7901/ADM cells by lipofection and electroporation methods ,respectively .The survival rates and transfection efficiency were analyzed .Results The efficiency of eGFP vector transfected into HepG2 cells by lipofection was （23 .8 ± 2 .1）% , compared with lipofection method ,the efficiency of eGFP plasmid transfected by electroporation was up to （49 .6 ± 2 .5）% ,and the difference was statistically significant（P〈0 .05） .The efficiency of SGC7901/ADM cells by lipofection was （25 .4 ± 1 .3）% ,com‐pared with lipofection method ,the efficiency of electroporation was up to（52 .6 ± 2 .1）% ,and the difference was statistically signifi‐cant（P〈0 .05） .This study provides reliable test parameters for electransfection of HepG2 and SGC7901/ADM cells .Conclusion The transfection efficiency of large fragment vector is efficiently improved by electroporation .

关 键 词：电穿孔 脂质体转染 HepG2细胞 SGC7901/ADM细胞 PCDNA3 .1-EGFP 

分 类 号：R394[医药卫生—医学遗传学]